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  1. No Access

    Protocol

    Characterization of N-Linked Glycosylation on Recombinant Glycoproteins Produced in Pichia pastoris Using ESI-MS and MALDI-TOF

    The production of recombinant therapeutic glycoproteins is an active area of research and drug development. Typically, improvements in therapeutic glycoprotein efficacy have focused on engineering additional N...

    Bing Gong, Michael Cukan, Richard Fisher, Huijuan Li, Terrance A. Stadheim… in Glycomics (2009)

  2. No Access

    Article

    Recombinant human lactoferrin expressed in glycoengineered Pichia pastoris: effect of terminal N-acetylneuraminic acid on in vitro secondary humoral immune response

    Traditional production of therapeutic glycoproteins relies on mammalian cell culture technology. Glycoproteins produced by mammalian cells invariably display N-glycan heterogeneity resulting in a mixture of gl...

    Byung-Kwon Choi, Jeffrey K. Actor, Sandra Rios, Marc d’Anjou… in Glycoconjugate Journal (2008)

  3. No Access

    Article

    Optimization of humanized IgGs in glycoengineered Pichia pastoris

    As the fastest growing class of therapeutic proteins, monoclonal antibodies (mAbs) represent a major potential drug class1. Human antibodies are glycosylated in their native state and all clinically approved mAbs...

    Huijuan Li, Natarajan Sethuraman, Terrance A Stadheim, Dongxing Zha… in Nature Biotechnology (2006)