18 Result(s)
within
Michael C. Flickinger
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Article
Biocoatings: challenges to expanding the functionality of waterborne latex coatings by incorporating concentrated living microorganisms
Biocoatings concentrate living, nongrowing microbes in nanoporous adhesive polymer films. Any microbial activity or trait of interest can be intensified and stabilized in biocoatings. These films will dramatic...
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Article
Characterization of Clostridium ljungdahlii OTA1: a non-autotrophic hyper ethanol-producing strain
A Clostridium ljungdahlii lab-isolated spontaneous-mutant strain, OTA1, has been shown to produce twice as much ethanol as the C. ljungdahlii ATCC 55383 strain when cultured in a mixotrophic medium containing fru...
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Article
Hybrid adsorbent nonwoven structures: a review of current technologies
Adsorptive nonwoven substrates are composite media that contain adsorbent materials within their fibrous structure; the methods to incorporate adsorbents in nonwoven webs determine their adsorption capacity. T...
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Article
Growth of Bacillus methanolicus in 2 M methanol at 50 °C: the effect of high methanol concentration on gene regulation of enzymes involved in formaldehyde detoxification by the ribulose monophosphate pathway
Bacillus methanolicus MGA3 is a Gram-positive aerobic methylotroph growing optimally at 50–53 °C. Methylotrophy in B. methanolicus is encoded on pBM19 and by two chromosomal copies of the methano...
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Article
A versatile method for preparation of hydrated microbial–latex biocatalytic coatings for gas absorption and gas evolution
We describe a latex wet coalescence method for gas-phase immobilization of microorganisms on paper which does not require drying for adhesion. This method reduces drying stresses to the microbes. It is applica...
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Protocol
Uniform Lab-Scale Biocatalytic Nanoporous Latex Coatings for Reactive Microorganisms
This chapter describes a method for generating uniform lab-scale biocatalytic nanoporous latex coatings. Nearly everything we come into contact with on a daily basis has been coated with some polymer material....
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Article
Thermodynamic Feasibility of Enzymatic Reduction of Carbon Dioxide to Methanol
Production of valuable chemicals from CO2 is highly desired for the purpose of controlling CO2 emission. Toward that, enzymatic reduction of CO2 for the production of methanol appeared to be especially promising....
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Article
Bacillus methanolicus pyruvate carboxylase and homoserine dehydrogenase I and II and their roles for l-lysine production from methanol at 50°C
We here present the pyc gene encoding pyruvate carboxylase (PC), and the hom-1 and hom-2 genes encoding two active homoserine dehydrogenase (HD) proteins, in methylotrophic Bacillus methanolicus MGA3. In general,...
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Article
Spatial expression of a mercury-inducible green fluorescent protein within a nanoporous latex-based biosensor coating
Optimizing the reactivity of cell coatings developed as biosensors or biocatalysts requires measurements of gene expression in the immobilized cells. To quantify and localize gene expression within a latex-bas...
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Article
Bacillus methanolicus: a candidate for industrial production of amino acids from methanol at 50°C
Amino acids are among the major products in biotechnology in both volume and value, and the global market is growing. Microbial fermentation is the dominant method used for industrial production, and today the...
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Article
Permeability and reactivity of Thermotoga maritima in latex bimodal blend coatings at 80°C: a model high temperature biocatalytic coating
Thermostable polymers cast as thin, porous coatings or membranes may be useful for concentrating and stabilizing hyperthermophilic microorganisms as biocatalysts. Hydrogel matricies can be unstable above 65°C....
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Article
Growth of Bacillus methanolicus in seawater-based media
Bacillus methanolicus has been proposed as a biocatalyst for the low cost production of commodity chemicals. The organism can use methanol as sole carbon and energy source, and it grows aerobically at elevated te...
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Article
Improved methods for investigating the external redox potential in hybridoma cell culture
Because of the interest in understanding and optimizing secretion of proteins from mammalian cells, reliable and more reproducible methods are needed to monitor the external redox potential of animal cells in ...
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Article
Monitoring growth and acetic acid secretion by a thermotolerantBacillus using conduction microcalorimetry
A method is described to determine power of heat-time curves by conduction microcalorimetry in order to monitor the viability and ability of a thermotolerantBacillus strain to secrete acetic acid both during expo...
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Article
Introduction
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Article
Methods for determination of growth-rate-dependent changes in hybridoma volume, shape and surface structure during continuous recycle
Hybridoma volume and surface membrane structure were found to vary as a function of specific growth rate using a method of cell recycle with continuous medium perfusion to vary growth rate. Mean hybridoma volu...
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Article
A novel acid proteinase relased by hybridoma cells
An acid proteinase has been detected in culture supernate of the 9.2.27 murine hybridoma. This enzyme extensively degrades albumin and transferrin during short incubations at pH 3 and below. Limited proteolysi...
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Chapter and Conference Paper
Conversion of hemicellulose carbohydrates
Hemicellulose can be converted to a variety of useful products. There are two approaches to hemicellulose bioconversion; hemicellulose can be directly converted, or the hemicellulose-derived carbohydrates can ...
