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Article
Open AccessReduced interleukin-18 secretion by human monocytic cells in response to infections with hyper-virulent Streptococcus pyogenes
Streptococcus pyogenes (group A streptococcus, GAS) causes a variety of diseases ranging from mild superficial infections of the throat and skin to severe invasive infections, such as necrotizing soft tissue infe...
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Article
Open AccessIntestinal stroma guides monocyte differentiation to macrophages through GM-CSF
Stromal cells support epithelial cell and immune cell homeostasis and play an important role in inflammatory bowel disease (IBD) pathogenesis. Here, we quantify the stromal response to inflammation in pediatri...
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Article
Open AccessNeutrophil-derived reactive agents induce a transient SpeB negative phenotype in Streptococcus pyogenes
Streptococcus pyogenes (group A streptococci; GAS) is the main causative pathogen of monomicrobial necrotizing soft tissue infections (NSTIs). To resist immuno-clearance, GAS adapt their genetic information and/o...
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Article
Open AccessInterplay between human STING genotype and bacterial NADase activity regulates inter-individual disease variability
Variability in disease severity caused by a microbial pathogen is impacted by each infection representing a unique combination of host and pathogen genomes. Here, we show that the outcome of invasive Streptococcu...
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Article
Open AccessTargeted plasma proteomics reveals signatures discriminating COVID-19 from sepsis with pneumonia
COVID-19 remains a major public health challenge, requiring the development of tools to improve diagnosis and inform therapeutic decisions. As dysregulated inflammation and coagulation responses have been impl...
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Chapter
Human Organotypic Respiratory Models
Biomedical research aiming to understand the molecular basis of human lung tissue development, homeostasis and disease, or to develop new therapies for human respiratory diseases, requires models that faithful...
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Article
Open AccessThe vitamin D analogue calcipotriol promotes an anti-tumorigenic phenotype of human pancreatic CAFs but reduces T cell mediated immunity
The pancreatic tumour stroma is composed of phenotypically heterogenous cancer-associated fibroblasts (CAFs) with both pro- and anti-tumorigenic functions. Here, we studied the impact of calcipotriol, a vitamin D
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Chapter
The INFECT-Project: An International and Multidisciplinary Project on Necrotizing Soft Tissue Infections
This book describes clinical and microbiologic aspects, pathogenesis, and diagnostics, related to the severe and rapidly spreading necrotizing soft tissue infections. The work has its foundation in a recently ...
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Chapter
Systems Biology and Biomarkers in Necrotizing Soft Tissue Infections
In necrotizing soft tissue infection (NSTI) there is a need to identify biomarker sets that can be used for diagnosis and disease management. The INFECT study was designed to obtain such insights through the i...
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Chapter
Pathogenic Mechanisms of Streptococcal Necrotizing Soft Tissue Infections
Necrotizing skin and soft tissue infections (NSTIs) are severe life-threatening and rapidly progressing infections. Beta-hemolytic streptococci, particularly S. pyogenes (group A streptococci (GAS)) but also S. d...
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Chapter
Novel Models to Study Stromal Cell-Leukocyte Interactions in Health and Disease
To study human immunology in general and stromal immunology in particular, it is highly motivated to move from monolayers to 3D cultures, such as organotypic models, that better mimic the function of living ti...
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Article
Open AccessAssociation between cytokine response, the LRINEC score and outcome in patients with necrotising soft tissue infection: a multicentre, prospective study
Early assessment of necrotising soft tissue infection (NSTI) is challenging. Analysis of inflammatory markers could provide important information about disease severity and guide decision making. For this purp...
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Article
Open AccessA point mutation in AgrC determines cytotoxic or colonizing properties associated with phenotypic variants of ST22 MRSA strains
Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of skin and soft tissue infections. One of the highly successful and rapidly disseminating clones is MRSA ST22 commonly associated with skin tro...
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Article
Open AccessIncreased cytotoxicity and streptolysin O activity in group G streptococcal strains causing invasive tissue infections
Streptococcus dysgalactiae subsp. equisimilis (SDSE) has emerged as an important cause of severe skin and soft tissue infections, but little is known of the pathogenic mechanisms underlying tissue pathology. Pati...
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Article
Open AccessNurture your scientific curiosity early in your research career
Uncertainty makes scientific research challenging and at the same time exciting. Whereas curiosity and passion for uncovering the unknown drive future generations of researchers, the landscape of science has c...
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Article
Open AccessPrime-Boost Vaccination with rBCG/rAd35 Enhances CD8+ Cytolytic T-Cell Responses in Lesions from Mycobacterium Tuberculosis-Infected Primates
To prevent the global spread of tuberculosis (TB) infection, a novel vaccine that triggers potent and long-lived immunity is urgently required. A plasmid-based vaccine has been developed to enhance activation ...
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Article
Photoinduced Charge Carriers in a Donor-Acceptor Double-Cable Polythiophene with Covalently Bound Tetracyanoanthraquinodimethane Moieties
The preparation of double-cable polymers, which consist of a hole conducting conjugated chain carrying pendant electron accepting/conducting moieties, is a promising strategy to prevent donor/acceptor phase segre...
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Protocol
Isolation and Culture of Human Hematopoietic Progenitors for Studies of Dendritic Cell Biology
Understanding the regulation of distinct dendritic cell (DC) function and differentiation pathways is important in many physiological and pathophysiological processes. This includes infectious and neoplastic d...
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Article
A Soluble Donor–Acceptor Double-Cable Polymer: Polythiophene with Pendant Fullerenes
We report the synthesis and the spectroscopic properties of a novel soluble thiophene-based copolymer carrying pendant fullerene moieties (donor–acceptor double-cable polymer). Photoinduced absorption (PIA) e...
