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  1. No Access

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    Scarless Recombineering of Phage in Lysogenic State

    We present a scarless recombineering-based method for introducing multiple point mutations into the genome of a temperate phage. The method uses the λ Red recombineering system to promote exogenous ssDNA oligo...

    Maria Ababi, Matthew Tridgett, Alexander Osgerby, Alfonso Jaramillo in Recombineering (2022)

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    Lambda Red Recombineering of Bacteriophage in the Lysogenic State

    We present a recombineering-based method for editing the genome of a temperate phage. The method uses the lambda Red recombination system to edit the genome of a lysogenized host with a prophage compatible wit...

    Matthew Tridgett, Maria Ababi, Alfonso Jaramillo in Recombineering (2022)