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Scarless Recombineering of Phage in Lysogenic State
We present a scarless recombineering-based method for introducing multiple point mutations into the genome of a temperate phage. The method uses the λ Red recombineering system to promote exogenous ssDNA oligo...
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Protocol
Lambda Red Recombineering of Bacteriophage in the Lysogenic State
We present a recombineering-based method for editing the genome of a temperate phage. The method uses the lambda Red recombination system to edit the genome of a lysogenized host with a prophage compatible wit...