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Protocol
Structural Probing of RNA Thermosensors
Chemical probing of RNA structure has become one of the most popular approaches to map the conformation of RNA molecules of various sizes under well-defined experimental conditions. The method monitors the sen...
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Article
More than one way to skin a cat: translational autoregulation by ribosomal protein S15
Some ribosomal proteins recognize both ribosomal RNA and their own messenger RNA similarly. Although this phenomenon is widely conserved among prokaryotes for ribosomal protein S15, the precise manner in which...
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Article
Structural basis of translational control by Escherichia coli threonyl tRNA synthetase
Escherichia coli threonyl-tRNA synthetase (ThrRS) represses the translation of its own messenger RNA by binding to an operator located upstream of the initiation codon. The crystal structure of the complex betwee...
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Article
Strains isogenic to S288C used in the Yeast Genome Sequencing Programme carry a functional KSS1 gene
In Saccharomyces cerevisiae, the KSS1 gene encodes the MAP kinase of the invasive/filamentous growth pathway. In addition to its role in this signal transduction pathway, Kss1 can replace the Fus3 MAP kinase in ...
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Article
Physiological effects of translation initiation factor IF3 and ribosomal protein L20 limitation inEscherichia coli
To investigate the physiological roles of translation initiation factor IF3 and ribosomal protein L20 inEscherichia coli, theinfC, rpmI andrpIT genes encoding IF3, L35 and L20, respectively, were placed under the...
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Chapter
Translational Control of Gene Expression in E. Coli and Bacteriophage
Gene expression can be regulated in response to very different stimuli. These may be external, such as the cellular growth medium, or internal, in response to a specific need at a given stage of the cell cycle...
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Article
CNRS defended
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Article
Translational control inE. coli: The case of threonyl-tRNA synthetase
Genetic studies have shown that expression of theE. coli threonyl-tRNA synthetase (thrS) gene is negatively auto-regulated at the translational level. A region called the operator, located 110 nucleotides downstr...
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Chapter
Escherichia coli Threonyl-Transfer RNA Synthetase as a Model System to Study Translational Autoregulation in Prokaryotes
For many years, it was believed that the regulation of gene expression in prokaryotes occurs only at the transcriptional level. However, many results in the last ten years demonstrate that the process of trans...
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Chapter and Conference Paper
Role of Messenger RNA Specific Secondary Structure in the Control of Gene Expression
Prokaryotic messenger RNA can form specific secondary structures involved in transcription termination. These structures can be located within as well as at the end of an operon. In the case of several aminoac...