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Protocol
How to Generate a Vascular-Labelled Transgenic Zebrafish Model to Study Tumor Angiogenesis and Extravasation
The use of transgenic animals carrying exogenous DNA integrated in their genome is a routine in modern-day laboratories. Nowadays, the zebrafish system represents the most useful tool for transgenesis studies ...
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Article
YAP/TAZ–TEAD link angiogenesis to nutrients
Ong et al. uncover a role for the YAP/TAZ–TEAD transcriptional pathways in retinal angiogenesis via the regulation of amino acid transporters and assessed mTORC activation. These findings establish the mechani...
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Article
Oxidative pentose phosphate pathway controls vascular mural cell coverage by regulating extracellular matrix composition
Vascular mural cells (vMCs) play an essential role in the development and maturation of the vasculature by promoting vessel stabilization through their interactions with endothelial cells. Whether endothelial ...
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Article
Open AccessLoss of pyruvate kinase M2 limits growth and triggers innate immune signaling in endothelial cells
Despite their inherent proximity to circulating oxygen and nutrients, endothelial cells (ECs) oxidize only a minor fraction of glucose in mitochondria, a metabolic specialization that is poorly understood. Her...
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Article
Open AccessBlood flow controls bone vascular function and osteogenesis
While blood vessels play important roles in bone homeostasis and repair, fundamental aspects of vascular function in the skeletal system remain poorly understood. Here we show that the long bone vasculature ge...
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Article
Open AccessPPFIA1 drives active α5β1 integrin recycling and controls fibronectin fibrillogenesis and vascular morphogenesis
Basolateral polymerization of cellular fibronectin (FN) into a meshwork drives endothelial cell (EC) polarity and vascular remodelling. However, mechanisms coordinating α5β1 integrin-mediated extracellular FN ...
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Protocol
Transgenic Zebrafish
The generation and use of transgenic animals carrying exogenous pieces of DNA stably integrated in their genome is a quite common practice in modern laboratories. Zebrafish have been increasingly used for tran...
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Article
ROS signaling and redox biology in endothelial cells
The purpose of this review is to provide an overview of redox mechanisms, sources and antioxidants that control signaling events in ECs. In particular, we describe which molecules are involved in redox signali...
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Article
13C-isotope-based protocol for prenyl lipid metabolic analysis in zebrafish embryos
Metabolism has a decisive role in many fundamental biological processes, including organism development and tissue homeostasis. Here we describe a protocol for fast and reliable 13C-isotope-based in vivo metaboli...
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Chapter
Tumor Angiogenesis: Fishing for Screening Models
The process of de novo vessel formation, called angiogenesis, is essential for tumor progression and metastasis. The identification and targeting of the molecular pathways involved in this process are becoming...
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Article
IAPs contain an evolutionarily conserved ubiquitin-binding domain that regulates NF-κB as well as cell survival and oncogenesis
A ubiquitin binding domain in the IAP proteins binds Lys 63-linked poly-ubiquitin chains and is essential for the oncogenic potential of cIAP. This domain is also required for activating NF-κB, possibly by bin...
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Article
Birc2 (cIap1) regulates endothelial cell integrity and blood vessel homeostasis
Integrity of the blood vessel wall is essential for vascular homeostasis and organ function1,2. A dynamic balance between endothelial cell survival and apoptosis contributes to this integrity during vascular deve...
