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Article
Role of the rice transcription factor JAmyb in abiotic stress response
Plants have developed certain adaptive responses to environmental stresses that cause adverse effects on growth. To identify genes involved in the adaptive mechanisms, we constructed a large population of tran...
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Article
rpoD gene expression as an indicator of bacterial pathogens in host plants
Pseudomonas, Ralstonia, and Xanthomonas are important genera of plant pathogenic bacteria that affect commercially cultivated crops. We developed an assay to assess the viability and development of bacterial path...
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Article
Monitoring fungal viability and development in plants infected with Colletotrichum higginsianum by quantitative reverse transcription-polymerase chain reaction
Colletotrichum higginsianum causes typical anthracnose lesions on a wide range of cruciferous plants. Currently, assays for C. higginsianum infection of host plants rely on visual diagnosis of symptoms. In this s...
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Article
Overexpression of a rice gene encoding a small C2 domain protein OsSMCP1 increases tolerance to abiotic and biotic stresses in transgenic Arabidopsis
Plant growth and crop production are limited by environmental stress. We used a large population of transgenic Arabidopsis expressing rice full-length cDNAs to isolate the rice genes that improve the tolerance...
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Article
Tolerance to various environmental stresses conferred by the salt-responsive rice gene ONAC063 in transgenic Arabidopsis
Environmental stresses limit plant growth and crop production worldwide. We attempted to isolate rice genes involved in conferring tolerance to environmental stresses by using a transgenic Arabidopsis populati...
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Article
Expression of rice heat stress transcription factor OsHsfA2e enhances tolerance to environmental stresses in transgenic Arabidopsis
Plant growth and crop yields are limited by high-temperature stresses. In this study, we attempted to isolate the rice genes responsible for high-temperature stress tolerance using a transformed Arabidopsis popul...
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Article
Characterization, cloning, sequencing, and expression of an aminopeptidase N from Streptomyces sp. TH-4
The aminopeptidase N (TH-4AP) of Streptomyces sp. TH-4 was purified from a culture supernatant. The purified enzyme had a molecular mass of 95 kDa. The gene encoding TH-4AP was cloned and sequenced. The primary s...
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Article
Assessment of ribozyme-mediated gene suppression using a luciferase reporter gene containing the sequence of Chrysanthemum Stunt Viroid as a target
Transgenic tobacco plants expressing hammerhead ribozyme (Rz), which specifically cleaves Chrysanthemum Stunt Viroid (CSVd) RNA, were produced via Agrobacterium-mediated transformation. To analyze the effects of ...
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Article
Study on peptide hydrolysis by aminopeptidases from Streptomyces griseus, Streptomyces septatus and Aeromonas proteolytica
We developed a spectrophotometric assay for peptide hydrolysis by aminopeptidases (APs). The assay enables the measurement of free amino acids liberated by AP-catalyzed peptide hydrolysis using 4-aminoantipyri...
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Article
Regulation of histone gene expression during the cell cycle
The steady-state level of histone mRNAs fluctuates coordinately with chromosomal DNA synthesis during the cell cycle. Such an S phase-specific expression pattern results from transcriptional activation of hist...
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Chapter
Regulation of histone gene expression during the cell cycle
The steady-state level of histone mRNAs fluctuates coordinately with chromosomal DNA synthesis during the cell cycle. Such an S phase-specific expression pattern results from transcriptional activation of hist...
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Article
Frontier of plant biology
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Article
S phase-specific expression of plant histone genes
The Oct motif (CGCGGATC) is highly conserved in plant histone gene promoters. Three types of Oct-containing composite elements have been identified: type I element composed of Hex and reverse-oriented Oct (CCACGT...
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Article
The flowering-time geneFT and regulation of flowering inArabidopsis
Transition from vegetative to reproductive development (flowering) is one of the most important decisions during the post-embryonic development of flowering plants. More than twenty loci are known to regulate ...
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Article
Conserved Ser residues in the basic region of the bZIP-type transcription factor HBP-1a(17): importance in DNA binding and possible targets for phosphorylation
HBP-1a(17) is representative of a group of plant bZIP-type transcription factors which includes HBP-1a proteins and G-box-binding factors. We found kinase activity in wheat nuclear extract that phosphorylated ...
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Article
Structural characteristics of two wheat histone H2A genes encoding distinct types of variants and functional differences in their promoter activity
To investigate the regulation of plant histone H2A gene expression, we isolated two H2A genes (TH254 and TH274) from wheat, which encode two variants of H2A. Both genes had an intron in the coding region. In t...
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Article
Structural and functional characterization of two wheat histone H2B promoters
Two wheat histone H2B genes (TH123 and TH153) were isolated. Nucleotide sequence analysis revealed that some characteristic sequence motifs were conserved in both the 5′- and 3′-flanking regions. A canonical T...
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Article
The historical differentiation process inHemerocallis middendorfii (Liliaceae) of Japan based on restriction site variations of chlorplast DNA
Chloroplast DNA restriction site variation inHemerocallis middendorfii Trautv. & Meyer was investigated to deduce the historical differentiation process that had taken place in populations among three districts H...
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Article
A type I element composed of the hexamer (ACGTCA) and octamer (CGCGGATC) motifs plays a role(s) in meristematic expression of a wheat histone H3 gene in transgenic rice plants
Type I element (CCACGTCACCGATCCGCG) is a well-conserved regulatory element found in proximal promoter region of a certain class of plant histone genes, that is composed of two independent cis-acting elements of t...
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Chapter
Gel mobility shift assay
Specific DNA-protein interactions are involved in many nuclear events such as transcription, replication and recombination. The first step towards the study on the specific DNA-protein interaction is to identi...
