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Article
Open AccessPhotoisomerization pathway of the microbial rhodopsin chromophore in solution
Photoisomerization is a key photochemical reaction in microbial and animal rhodopsins. It is well established that such photoisomerization is highly selective; all-trans to 13-cis, and 11-cis to all-trans forms i...
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Article
Processing of the 5′-UTR and existence of protein factors that regulate translation of tobacco chloroplast psbN mRNA
The chloroplast psbB operon includes five genes encoding photosystem II and cytochrome b 6 /f complex components. The psbN gene is located on the opposite strand. PsbN is localized...
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Protocol
Plastid mRNA Translation
Overall translational machinery in plastids is similar to that of E. coli. Initiation is the crucial step for translation and this step in plastids is somewhat different from that of E. coli. Unlike the Shine-Dal...
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Article
Complete nucleotide sequence of the freshwater unicellular cyanobacterium Synechococcus elongatus PCC 6301 chromosome: gene content and organization
The entire genome of the unicellular cyanobacterium Synechococcus elongatus PCC 6301 (formerly Anacystis nidulans Berkeley strain 6301) was sequenced. The genome consisted of a circular chromosome 2,696,255 bp lo...
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Article
The 2005 version of the chloroplast DNA sequence from tobacco (Nicotiana tabacum)
The complete nucleotide sequence of tobacco chloroplast DNA was first determined in 1986, and then its updated gene map was reported in 1998. During the course of sequencing the chloroplast DNA ofNicotiana sylves...
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Chapter
History of chloroplast genomics
The presence of chloroplast DNA was established in 1963. With the development of recombinant DNA technologies, chloroplast DNA was selected as one of the first candidates for genome sequencing. The first physi...
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Chapter
In Vitro Transcription Systems from BY-2 Cells
The in vitro transcription system is a powerful method to dissect molecular mechanisms of transcription of genes in question, usually performed in cellular or nuclear extracts containing transcription apparatu...
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Article
History of chloroplast genomics
The presence of chloroplast DNA was established in 1963. With the development of recombinant DNA technologies, chloroplast DNA was selected as one of the first candidates for genome sequencing. The first physi...
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Article
Plant 7SL RNA and tRNATyr genes with inserted antisense sequences are efficiently expressed in an in vitro transcription system from Nicotiana tabacum cells
RNA polymerase III-driven cassettes for the expression of antisense RNAs and ribozymes have recently attracted much attention because (1) pol III genes are transcribed abundantly in all kinds of tissues and (2...
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Article
Comparative Analysis of RNA Editing Sites in Higher Plant Chloroplasts
Transcripts of land plant chloroplast genomes undergo C-to-U RNA editing. Systematic search disclosed 31 editing sites in tobacco, 27 in maize, and 21 in rice. Based on these identified sites, potential editi...
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Article
The genomics of land plant chloroplasts: Gene content and alteration of genomic information by RNA editing
The entire nucleotide sequence of the chloroplast genome has been determined from 12 land plants. The gene content and arrangement are relatively uniform from species to species, and the genome contains an ave...
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Article
Splicing of Arabidopsis tRNAMet precursors in tobacco cell and wheat germ extracts
Intron-containing tRNA genes are exceptional within nuclear plant genomes. It appears that merely two tRNA gene families coding for tRNATyr GΨ A and elongator tRNAMet CmAU contain intervening sequences. We have p...
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Chapter
Structure and Expression of LHCB Genes from Nicotiana Sylvestris
Light harvesting chlorophyll a/b-binding proteins, Lhca and Lhcb, are major components of antenna complexes in PSI and PSII, respectively. Lhca and Lhcb proteins are encoded by the nuclear genes, synthesized as p...
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Chapter
The Function of the Cyanobacterial RNA-Binding Proteins
Posttranscriptional and translational regulation of gene expression in cyanobacteria have recently been reported (1-3). The different stability of mRNAs is suggested to have an important role for the regulatio...
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Chapter
Characterization of Ribonucleoproteins from the Tobacco Chloroplast
Tobacco chloroplasts contain five distinct RNA-binding proteins, cp28 to cp33, which consist of an acidic N-terminal domain and two RNA-binding domains (1, 2). Spinach 28 kDa protein, 28RNP, similar to the tob...
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Chapter
In Vitro Transcription Analysis of the rbcS Gene
Photosynthetic gene expression is a most significant theme of plant molecular biology. Until now, many researcher have approached to make clear this problem from different aspects. Especially light-dependent g...
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Article
Regulation of gene expression in chloroplasts of higher plants
Chloroplasts contain their own genetic system which has a number of prokaryotic as well as some eukaryotic features. Most chloroplast genes of higher plants are organized in clusters and are cotranscribed as p...
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Article
Physical and gene maps of the unicellular cyanobacterium Synechococcus sp. strain PCC6301 genome
A physical map of the unicellular cyanobacterium Synechococcus sp. strain PCC6301 genome has been constructed with restriction endonucleases PmeI, SwaI, and an intron-encoded endonuclease I-CeuI. The estimated si...
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Article
Isolation of a novel RNA-binding protein and its association with a large ribonucleoprotein particle present in the nucleoplasm of tobacco cells
A cDNA encoding a protein with a consensus sequence-type RNA-binding domain (CS-RBD) has been isolated from a Nicotiana sylvestris cDNA library. The deduced protein (designated ‘RZ-1’) contains CS-RBD in its N-te...
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Article
Occurrence of silent RNA editing in chloroplasts: its species specificity and the influence of environmental and developmental conditions
We have identified three new C-to-U RNA editing sites, one in atpF and two in atpA transcripts from tobacco chloroplasts. Two of them lead to amino acid substitutions to restore the conserved amino acid found in ...