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Article
Open AccessComparative analysis demonstrates cell type-specific conservation of SOX9 targets between mouse and chicken
SRY (sex-determining region Y)-box 9 (SOX9) is a transcription factor regulating both chondrogenesis and sex determination. Among vertebrates, SOX9’s functions in chondrogenesis are well conserved, while they ...
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Article
Creation of CRISPR-based germline-genome-engineered mice without ex vivo handling of zygotes by i-GONAD
Methods to create genetically engineered mice involve three major steps: harvesting embryos from one set of females, microinjection of reagents into embryos ex vivo and their surgical transfer to another set o...
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Article
Open AccessWwp2 maintains cartilage homeostasis through regulation of Adamts5
The WW domain-containing protein 2 (Wwp2) gene, the host gene of miR-140, codes for the Wwp2 protein, which is an HECT-type E3 ubiquitin ligases abundantly expressed in articular cartilage. However, its function ...
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Article
Dissecting the roles of miR-140 and its host gene
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Article
Open AccessThe p.R92W variant of NR5A1/Nr5a1 induces testicular development of 46,XX gonads in humans, but not in mice: phenotypic comparison of human patients and mutation-induced mice
NR5A1 is the key regulator of adrenal and gonadal development in both humans and mice. Recently, a missense substitution in human NR5A1, p.R92W, was shown to underlie gonadal dysgenesis in genetic males and testi...
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Article
Open AccessMohawk promotes the maintenance and regeneration of the outer annulus fibrosus of intervertebral discs
The main pathogenesis of intervertebral disc (IVD) herniation involves disruption of the annulus fibrosus (AF) caused by ageing or excessive mechanical stress and the resulting prolapse of the nucleus pulposus...
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Article
Open AccessGeneration of mutant mice via the CRISPR/Cas9 system using FokI-dCas9
Genome editing, which introduces mutations in genes of interest using artificial DNA nucleases such as the ZFN, TALEN and CRISPR/Cas9 systems in living cells, is a useful tool for generating mutant animals. Al...
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Article
Open AccessRapid generation of mouse models with defined point mutations by the CRISPR/Cas9 system
Introducing a point mutation is a fundamental method used to demonstrate the roles of particular nucleotides or amino acids in the genetic elements or proteins and is widely used in in vitro experiments based on ...
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Article
Open AccessProduction of Sry knockout mouse using TALEN via oocyte injection
Recently developed transcription activator-like effector nuclease (TALEN) technology has enabled the creation of knockout mice, even for genes on the Y chromosome. In this study, we generated a knockout mouse ...
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Article
USP15 is a deubiquitylating enzyme for receptor-activated SMADs
In the TGFβ pathway, receptor-activated SMADs (R-SMADs) associate with SMAD4 to regulate transcription. Piccolo and colleagues reveal that the deubiquitylase USP15 is required for TGFβ responses by reversing R...
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Article
MicroRNA control of signal transduction
Signal transduction pathways are prime candidates for microRNA (miRNA)-mediated regulation: the highly dynamic and dose-sensitive signalling complexes are the ...
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Article
MicroRNA control of Nodal signalling
MicroRNAs are crucial modulators of gene expression, yet their involvement as effectors of growth factor signalling is largely unknown. Ligands of the transforming growth factor-β superfamily are essential for...
