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Article
Open AccessZebrafish Avatar-test forecasts clinical response to chemotherapy in patients with colorectal cancer
Cancer patients often undergo rounds of trial-and-error to find the most effective treatment because there is no test in the clinical practice for predicting therapy response. Here, we conduct a clinical study...
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Protocol
Patient-Derived Breast Cancer Tissue Cultures for Anti-Endocrine Drug Assays
cancer is a complex and heterogeneous pathology, characterized by a variety of histological and molecular phenotypes. The majority of the breast cancers express the estrogen receptor alpha (ER), which plays a...
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Article
Open AccessA novel culture method that sustains ERα signaling in human breast cancer tissue microstructures
Estrogen receptor α (ERα) signaling is a defining and driving event in most breast cancers; ERα is detected in malignant epithelial cells of 75% of all breast cancers (classified as ER-positive breast cancer) ...
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Chapter
In Vitro and Ex Vivo Models – The Tumor Microenvironment in a Flask
Experimental tumor modeling has long supported the discovery of fundamental mechanisms of tumorigenesis and tumor progression, as well as provided platforms for the development of novel therapies. Still, the a...
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Protocol
PREDECT Protocols for Complex 2D/3D Cultures
PREDECT, a European IMI consortium, has assumed the task to generate robust 2D and 3D culture platforms. Protocols established for 2D and 3D monoculture and stromal coculture models of increasing complexity (s...
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Article
Open AccessProtocols and characterization data for 2D, 3D, and slice-based tumor models from the PREDECT project
Two-dimensional (2D) culture of cancer cells in vitro does not recapitulate the three-dimensional (3D) architecture, heterogeneity and complexity of human tumors. More representative models are required that bett...
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Article
Open AccessCapturing tumor complexity in vitro: Comparative analysis of 2D and 3D tumor models for drug discovery
Two-dimensional (2D) cell cultures growing on plastic do not recapitulate the three dimensional (3D) architecture and complexity of human tumors. More representative models are required for drug discovery and ...
