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Article
Open AccessEnabling low cost biopharmaceuticals: high level interferon alpha-2b production in Trichoderma reesei
The filamentous fungus Trichoderma reesei has tremendous capability to secrete over 100 g/L of proteins and therefore it would make an excellent host system for production of high levels of therapeutic proteins a...
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Article
Open AccessThe effects of extracellular pH and of the transcriptional regulator PACI on the transcriptome of Trichoderma reesei
Extracellular pH is one of the several environmental factors affecting protein production by filamentous fungi. Regulatory mechanisms ensure that extracellular enzymes are produced under pH-conditions in which...
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Article
Open AccessComparison of intracellular and secretion-based strategies for production of human α-galactosidase A in the filamentous fungus Trichoderma reesei
Trichoderma reesei is known as a good producer of industrial proteins but has hitherto been less successful in the production of therapeutic proteins. In order to elucidate the bottlenecks of heterologous protein...
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Article
Open AccessRe-annotation of the CAZy genes of Trichoderma reesei and transcription in the presence of lignocellulosic substrates
Trichoderma reesei is a soft rot Ascomycota fungus utilised for industrial production of secreted enzymes, especially lignocellulose degrading enzymes. About 30 carbohydrate active enzymes (CAZymes) of T. reesei ...
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Article
Open AccessThe effects of disruption of phosphoglucose isomerase gene on carbon utilisation and cellulase production in Trichoderma reesei Rut-C30
Cellulase and hemicellulase genes in the fungus Trichoderma reesei are repressed by glucose and induced by lactose. Regulation of the cellulase genes is mediated by the repressor CRE1 and the activator XYR1. T. r...
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Article
Open AccessProtein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview
Different species of microorganisms including yeasts, filamentous fungi and bacteria have been used in the past 25 years for the controlled production of foreign proteins of scientific, pharmacological or indu...
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Article
Open AccessPhysiological evaluation of the filamentous fungus Trichoderma reesei in production processes by marker gene expression analysis
Biologically relevant molecular markers can be used in evaluation of the physiological state of an organism in biotechnical processes. We monitored at high frequency the expression of 34 marker genes in batch,...
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Article
Open AccessHeterologous expression of isotopically labeled Trichoderma reesei tyrosinase 2 in Pichia pastoris
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Open AccessGenome-wide analysis of protein production physiology in the filamentous fungus Trichoderma reesei
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Article
Open AccessMonitoring of transcript regulation and protein production related stress responses in Pichia pastoris secreting Fab antibody fragments