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  1. No Access

    Article

    Expression of Two Novel β-Glucosidases from Chaetomium atrobrunneum in Trichoderma reesei and Characterization of the Heterologous Protein Products

    Two novel GH3 family thermostable β-glucosidases from the filamentous fungus Chaetomium atrobrunneum (CEL3a and CEL3b) were expressed in Trichoderma reesei, purified by two-step ion exchange chromatography, and c...

    Ana C. Colabardini, Mari Valkonen, Anne Huuskonen… in Molecular Biotechnology (2016)

  2. Article

    Open Access

    Exploring laccase-like multicopper oxidase genes from the ascomycete Trichoderma reesei: a functional, phylogenetic and evolutionary study

    The diversity and function of ligninolytic genes in soil-inhabiting ascomycetes has not yet been elucidated, despite their possible role in plant litter decay processes. Among ascomycetes, Trichoderma reesei is a...

    Anthony Levasseur, Markku Saloheimo, David Navarro, Martina Andberg… in BMC Biochemistry (2010)

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  3. Article

    Open Access

    Secreted fungal sulfhydryl oxidases: sequence analysis and characterisation of a representative flavin-dependent enzyme from Aspergillus oryzae

    Sulfhydryl oxidases are flavin-dependent enzymes that catalyse the formation of de novo disulfide bonds from free thiol groups, with the reduction of molecular oxygen to hydrogen peroxide. Sulfhydryl oxidases ...

    Greta Faccio, Kristiina Kruus, Johanna Buchert, Markku Saloheimo in BMC Biochemistry (2010)

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  4. No Access

    Article

    cDNA encoding protein O-mannosyltransferase from the filamentous fungus Trichoderma reesei; functional equivalence to Saccharomyces cerevisiae PMT2

    O-Mannosylation is suggested to be essential for protein secretion in Trichoderma reesei. In protein O-glycosylation, the first mannosyl residue is transferred to a serine or threonine hydroxyl group of the prot...

    Anna Zakrzewska, Andrzej Migdalski, Markku Saloheimo, Merja E. Penttila… in Current Genetics (2003)

  5. No Access

    Article

    Crystal structure of a laccase from Melanocarpus albomyces with an intact trinuclear copper site

    We have crystallized the ascomycete laccase from Melanocarpus albomyces with all four coppers present and determined the crystal structure at 2.4 Å resolution. The enzyme is heavily glycosylated and consists of t...

    Nina Hakulinen, Laura-Leena Kiiskinen, Kristiina Kruus… in Nature Structural Biology (2002)

  6. No Access

    Article

    Trichoderma reeseirho3, a homologue of yeast RHO3, suppresses the growth defect of yeast sec15-1 mutation

    The Trichoderma reesei gene, rho3, encoding the functional homologue of the Saccharomyces cerevisiae small GTP-binding protein Rho3p was cloned as a suppressor of the secretion-deficient mutation sec15-1 in yeas...

    Tuija Vasara, Markku Saloheimo, Sirkka Keränen, Merja Penttilä in Current Genetics (2001)

  7. No Access

    Article

    Isolation of a Trichoderma reesei cDNA encoding GTP: a-d-mannose-1-phosphate guanyltransferase involved in early steps of protein glycosylation

    A cDNA coding for GTP: α-d-mannose-1-phosphate guanyltransferase (MPG1 transferase) (EC 2.7.7.13) was isolated from a cDNA library of the Trichoderma reesei RutC-30 strain by suppression of the yeast Saccharomyc...

    Joanna S. Kruszewska, Markku Saloheimo, Merja Penttilä… in Current Genetics (1998)

  8. No Access

    Article

    Laccase from the white-rot fungus Trametes versicolor: cDNA cloning of lcc1 and expression in Pichia pastoris

    A cDNA coding for laccase was isolated from the ligninolytic fungus Trametes versicolor by RNA-PCR. The cDNA corresponds to the gene lcc1, which encodes a laccase isoenzyme of 498 amino-acid residues preceded by...

    L. J. Jönsson, Markku Saloheimo, Merja Penttilä in Current Genetics (1997)

  9. No Access

    Article

    Characterization of a birch (Betula pendula Roth.) embryogenic gene, BP8

    We have isolated the birch homologue (BP8) for the carrot embryogenic gene DC8 by heterologous hybridization. The birch BP8 gene encodes a putative protein of 53 kDa, showing 52% sequence identity with the DC8 ge...

    Riitta Puupponen-Pimiä, Markku Saloheimo, Tuija Vasara, Runar Ra… in Plant Molecular Biology (1993)