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Article
Open AccessSARS-CoV-2-specific cellular and humoral immunity after bivalent BA.4/5 COVID-19-vaccination in previously infected and non-infected individuals
Knowledge is limited as to how prior SARS-CoV-2 infection influences cellular and humoral immunity after booster-vaccination with bivalent BA.4/5-adapted mRNA-vaccines, and whether vaccine-induced immunity may...
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Article
Open AccessPotent induction of humoral and cellular immunity after bivalent BA.4/5 mRNA vaccination in dialysis patients
Knowledge on immunogenicity of the bivalent Omicron BA.4/5 vaccine in dialysis patients and the effect of a previous infection is limited. Therefore, vaccine-induced humoral and cellular immunity was analyzed ...
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Article
Open AccessCytokine-responsive T- and NK-cells portray SARS-CoV-2 vaccine-responders and infection in multiple myeloma patients
Patients with multiple myeloma (MM) routinely receive mRNA-based vaccines to reduce COVID-19-related mortality. However, whether disease- and therapy-related alterations in immune cells and cytokine-responsive...
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Article
Open AccessEfficacy of an unmodified bivalent mRNA vaccine against SARS-CoV-2 variants in female small animal models
Combining optimized spike (S) protein-encoding mRNA vaccines to target multiple SARS-CoV-2 variants could improve control of the COVID-19 pandemic. We compare monovalent and bivalent mRNA vaccines encoding B.1...
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Article
Open AccessThe spike gene is a major determinant for the SARS-CoV-2 Omicron-BA.1 phenotype
Variant of concern (VOC) Omicron-BA.1 has achieved global predominance in early 2022. Therefore, surveillance and comprehensive characterization of Omicron-BA.1 in advanced primary cell culture systems and ani...
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Article
Reduced interferon antagonism but similar drug sensitivity in Omicron variant compared to Delta variant of SARS-CoV-2 isolates
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Article
Induction of robust cellular and humoral immunity against SARS-CoV-2 after a third dose of BNT162b2 vaccine in previously unresponsive older adults
Here we compared SARS-CoV-2-specific antibody and T-cell responses between older adults (>80 years old, n = 51) and a younger control group (20–53 years old, n = 46) after receiving two doses of BNT162b2. We foun...
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Protocol
Detection and Quantification of SARS-CoV-2 by Real-Time RT-PCR Assay
The pandemic coronavirus SARS-CoV-2 is the causative agent of the acute respiratory disease COVID-19, which has become a global concern due to its rapid spread and high mortality rate. Detection and quantifica...
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Article
Open AccessEvaluation of stability and inactivation methods of SARS-CoV-2 in context of laboratory settings
The novel coronavirus SARS-CoV-2 is the causative agent of the acute respiratory disease COVID-19, which has become a global concern due to its rapid spread. Laboratory work with SARS-CoV-2 in a laboratory set...
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Article
Papain-like protease regulates SARS-CoV-2 viral spread and innate immunity
The papain-like protease PLpro is an essential coronavirus enzyme that is required for processing viral polyproteins to generate a functional replicase complex and enable viral spread1,2. PLpro is also implicated...
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Article
Proteomics of SARS-CoV-2-infected host cells reveals therapy targets
A new coronavirus was recently discovered and named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Infection with SARS-CoV-2 in humans causes coronavirus disease 2019 (COVID-19) and has been rap...
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Article
Open AccessConcurrent administration of IFNα14 and cART in TKO-BLT mice enhances suppression of HIV-1 viremia but does not eliminate the latent reservoir
Combination antiretroviral therapy (cART) prevents HIV-1 replication but does not eliminate the latent reservoir and cure the infection. Type I interferons (IFN) mediate antiviral effects through different mec...
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Article
Open AccessHIV-1 persistent viremia is frequently followed by episodes of low-level viremia
After the start of antiretroviral therapy (ART), plasma HIV-RNA levels should fall below the limit of detection (LOD) within 24 weeks. Hence, the prolonged decline of HIV-RNA after ART initiation is defined as...
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Article
Open AccessBalanced splicing at the Tat-specific HIV-1 3′ss A3 is critical for HIV-1 replication
The viral regulatory protein Tat is essential for establishing a productive transcription from the 5′-LTR promoter during the early phase of viral gene expression. Formation of the Tat-encoding mRNAs requires ...
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Article
Open AccessA functional conserved intronic G run in HIV-1 intron 3 is critical to counteract APOBEC3G-mediated host restriction
The HIV-1 accessory proteins, Viral Infectivity Factor (Vif) and the pleiotropic Viral Protein R (Vpr) are important for efficient virus replication. While in non-permissive cells an appropriate amount of Vif ...
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Article
Open AccessThe D-amino acid peptide D3 reduces amyloid fibril boosted HIV-1 infectivity
Amyloid fibrils such as Semen-Derived Enhancer of Viral Infection (SEVI) or amyloid-β-peptide (Aβ) enhance HIV-1 attachment and entry. Inhibitors destroying or converting those fibrils into non-amyloidogenic a...
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Open AccessHEXploring of the HIV-1 genome allows landsca** of new potential splicing regulatory elements
