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  1. Article

    Open Access

    Skeletal muscle metabolomics and blood biochemistry analysis reveal metabolic changes associated with dietary amino acid supplementation in dairy calves

    The effects of different amino acid (AA) supplementations of milk protein-based milk replacers in pre-ruminant calves from 3 days to 7 weeks of age were studied. Animals were divided into 4 groups: Ctrl) Contr...

    Kuai Yu, Manolis Matzapetakis, Daniel Valent, Yolanda Saco… in Scientific Reports (2018)

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  2. No Access

    Chapter

    NMR Metabolomics pari passu with Proteomics: Two Relevant Tools for Animal Sciences Combined

    Metabolomics is a field of growing importance in farm animal science and agricultural research. It may be based on either mass spectrometry or NMR (nucleic magnetic resonance) spectroscopy and is particularly ...

    Mariana Palma, André M. de Almeida… in Proteomics in Domestic Animals: from Farm … (2018)

  3. Article

    Open Access

    The hepatic and skeletal muscle ovine metabolomes as affected by weight loss: a study in three sheep breeds using NMR-metabolomics

    Sheep are a valuable resource for meat and wool production. During the dry summer, pastures are scarce and animals face Seasonal Weight Loss (SWL), which decreases production yields. The study of breeds tolera...

    Mariana Palma, Tim Scanlon, Tanya Kilminster, John Milton… in Scientific Reports (2016)

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  4. No Access

    Article

    Chemical shift assignments and secondary structure determination of the ectodomain of Bacillus subtilis morphogenic protein RodZ

    RodZ (also known as YfgA) is a component of the core bacterial morphogenic apparatus. RodZ is a key cell shape determinant in rod-shaped bacteria and it interacts with the actin-like cytoskeletal protein MreB....

    Ana C. Pereira, Ana Paiva, Ivo H. Saraiva, Teresa Costa… in Biomolecular NMR Assignments (2015)

  5. No Access

    Article

    1H, 13C and 15N resonance assignment of the soluble form of the Lipid-modified Azurin from Neisseria gonorrhoeae

    Lipid-modified azurin (Laz) from Neisseria gonorrhoeae is a type 1 copper protein proposed to be the electron donor to several enzymes involved in the resistance mechanism to reactive oxygen and nitrogen species....

    Cláudia S. Nóbrega, Manolis Matzapetakis, Sofia R. Pauleta in Biomolecular NMR Assignments (2013)

  6. No Access

    Article

    Backbone and side chain 1H, 15N and 13C assignments for a thiol-disulphide oxidoreductase from the Antarctic bacterium Pseudoalteromonas haloplanktis TAC125

    Enzymes produced by psychrophilic organisms have successfully overcome the low temperature challenge and evolved to maintain high catalytic rates in their permanently cold environments. As an initial step in o...

    Tony Collins, Manolis Matzapetakis, Helena Santos in Biomolecular NMR Assignments (2010)

  7. No Access

    Article

    13C–13C NOESY spectra of a 480 kDa protein: solution NMR of ferritin

    Molecular size has limited solution NMR analyses of proteins. We report 13C–13C NOESY experiments on a 480 kDa protein, the multi-subunit ferritin nanocage with gated pores. By exploiting 13C-resonance-specific c...

    Manolis Matzapetakis, Paola Turano, Elizabeth C. Theil… in Journal of Biomolecular NMR (2007)

  8. No Access

    Article

    Ferritins: iron/oxygen biominerals in protein nanocages

    Ferritin protein nanocages that form iron oxy biominerals in the central nanometer cavity are nature’s answer to managing iron and oxygen; gene deletions are lethal in mammals and render bacteria more vulnerab...

    Elizabeth C. Theil, Manolis Matzapetakis… in JBIC Journal of Biological Inorganic Chemi… (2006)

  9. No Access

    Article

    Peptidic models for the binding of Pb(II), Bi(III) and Cd(II) to mononuclear thiolate binding sites

    Herein, we evaluate the binding of Pb(II) and Bi(III) to cysteine-substituted versions of the TRI peptides [AcG-(LKALEEK)4G-NH2] which have previously been shown to bind Hg(II) and Cd(II) in unusual geometries as...

    Manolis Matzapetakis, Debdip Ghosh… in JBIC Journal of Biological Inorganic Chemi… (2006)