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Article
Continued Development of the Urban Hassles Index: Evolving Examination of Urban Adolescent Stressors
The continued development of the Urban Hassles Index (UHI) as a tool for understanding the exposure of urban adolescents to chronic stressors is expanded to include the severity of each stressor. Co** strate...
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Article
Molecular systematics of Solanum section Lycopersicum (Lycopersicon) using the nuclear ITS rDNA region
The phylogenetic relationships of all nine known tomato species of Solanum section Lycopersicum, together with other Solanum sections and species from several related genera, were investigated using parsimony ana...
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Article
Aloe L. – a second plant family without (TTTAGGG)n telomeres
The physical ends of chromosomes are protected and stabilised by telomeres. The sequence of telomeric DNA normally consists of a simple repeating unit that is conserved in many organisms. Most plants examined ...
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Article
Homoeologous pairing and recombination in backcross derivatives of tomato somatic hybrids [Lycopersicon esculentum (+) L. peruvianum]
Genomic in situ hybridization (GISH) was used to examine genome interactions in two allohexa ploid (2n=6x=72) Lycopersicon esculentum (+) L. peruvianum somatic hybrids and their seed progenies originated from su...
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Article
The use of genomic in situ hybridization (GISH) to show transmission of recombinant chromosomes by a partially fertile bigeneric hybrid,Gasteria lutzii x Aloe aristata (Aloaceae), to its progeny
Genomic in situ hybridization (GISH) was used to study somatic chromosomes of parental and progeny plants (all 2n=2x=14) of the bigeneric hybrid betweenGasteria lutzii andAloe aristata (Aloaceae), which is partia...
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Article
The use of genomic in situ hybridization (GISH) to show transmission of recombinant chromosomes by a partially fertile bigeneric hybrid, Gasteria lutzii ×Aloe aristata (Aloaceae), to its progeny
Genomic in situ hybridization (GISH) was used to study somatic chromosomes of parental and progeny plants (all 2n=2x=14) of the bigeneric hybrid between Gasteria lutzii and Aloe aristata (Aloaceae), which is part...
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Chapter
Cytological Characterization of Transformed Plants: Map** of Low-Copy and Repetitive DNA Sequences by Fluorescent In Situ Hybridization (FISH)
The physical localization of DNA sequences to plant chromosomes by in situ hybridization (ISH) is becoming increasingly important in many areas of plant molecular biology. ISH has provided new information on t...
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Chapter
New insights into chromosome evolution in plants from molecular cytogenetics
To understand the mechanisms involved in chromosome evolution, the genomes of many different organisms have been analysed. These analyses are regarded as fundamental to evolutionary problems because they detec...
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Chapter
Cytological Characterization of Somatic Hybrids: Detection of Genome Origin by Genomic In Situ Hybridization (GISH)
Genomic in situ hybridization (GISH) allows genomes differing in molecular composition to be discriminated visually in cell nuclei. Total genomic DNA from a given genotype (e.g. AA) is used to probe for chromosom...
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Article
Chromosomal location of endogenous geminivirus-related DNA sequences inNicotiana tabacum L.
TheN. tabacum (tobacco) nuclear genome carries approximately 25 multiple direct repeats of a geminivirus-related DNA (GRD) sequence that probably arose by illegitimate recombination, following geminivrus infectio...
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Article
Chromosome identification and map** in the grassZingeria biebersteiniana (2n=4) using fluorochromes
The grassZingeria biebersteiniana is one of five angiosperms known with 2n=2x=4. Its chromosomes were studied using fluorochrome banding and fluorescencein situ hybridization (FISH). The large pericentromeric reg...
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Article
Evidence for somatic translocation during potato dihaploid induction
Potato dihaploid PDH55 (Solanum tuberosum) is exclusively euploid (2n = 24) but apparently contains and expresses DNA from dihaploid inducer IVP48 (S. phureja). Genomic in situ hybridization (GISH) suggested IVP4...
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Article
Durum wheat haploid production using maize wide-crossing
While anther culture or pollinations with Hordeum bulbosum have provided suitable methods for haploid production in bread wheat, they have been largely unsuccessful in durum wheat. Pollinations with maize were us...
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Article
The fate of recombinant chromosomes and genome interaction in Nicotiana asymmetric somatic hybrids and their sexual progeny
Genomic in-situ hybridization (GISH) was used to monitor the behaviour of parental genomes, and the fate of intergenomic chromosome translocations, through meiosis of plants regenerated from asymmetric somatic...
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Article
Parental genome separation and asynchronous centromere division in interspecific F1 hybrids inLathyrus
Chromosomes were studied in root-tip metaphase cells of several F1 interspecificLathyrus hybrids including:L. hirsutus × L. cassius (H × C),L. cassius × L. hirsutus (C × H),L. cassius × L. odoratus (C × O), and t...
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Article
Comparative responses of tetraploid wheats pollinated with Zea mays L. and Hordeum bulbosum L.
Ten different tetraploid wheat (Triticum turgidum) genotypes were pollinated with maize (Zea mays). Fertilization was achieved in all ten genotypes and no significant difference in fertilization frequency between...
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Article
Secondary constrictions and NORs of of Lathyrus investigated by silver staining and in-situ hybridization
A variety of karyotypic differences were seen among six Lathyrus species. In part these differences are the result of different numbers of metacentric and acrocentric chromosomes in the complement but there were ...
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Article
Parental genomes are separated throughout the cell cycle in a plant hybrid
The positions of the genomes originating from each parent were analysed in root-tip nuclei of the mature, sexual F1 hybrid plant Hordeum vulgare (barley) x Secale africanum (a wild rye). The two genomes of the hy...
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Article
Cytotaxonomic studies of Eastern MediterraneanVicia species (Leguminosae)
Collected material of rare and newVicia spp. was investigated from a cytotaxonomic point of view. Nineteen species were examined in total. The study includes 14 novel chromosome counts and measurements of DNA amo...
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Article
Discrimination between closely related Triticeae species using genomic DNA as a probe
Labelled total genomic DNA was used as a probe in combination with blocking DNA to discriminate between taxonomically closely related species in the genera Hordeum and Secale. Discrimination was possible both by ...
