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Protocol
Lattice Light-Sheet Motor-PAINT: A Method to Map the Orientations of Microtubules in Complex Three-Dimensional Arrays
Microtubules play an essential role in many cellular functions, in part by serving as tracks for intracellular transport by kinesin and dynein. The ability of microtubules to fulfill this role fundamentally de...
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Article
Open AccessGelMap: intrinsic calibration and deformation map** for expansion microscopy
Expansion microscopy (ExM) is a powerful technique to overcome the diffraction limit of light microscopy by physically expanding biological specimen in three dimensions. Nonetheless, using ExM for quantitative...
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Article
Mechanisms of microtubule organization in differentiated animal cells
Microtubules are polarized cytoskeletal filaments that serve as tracks for intracellular transport and form a scaffold that positions organelles and other cellular components and modulates cell shape and mecha...
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Article
Open AccessER – lysosome contacts at a pre-axonal region regulate axonal lysosome availability
Neuronal function relies on careful coordination of organelle organization and transport. Kinesin-1 mediates transport of the endoplasmic reticulum (ER) and lysosomes into the axon and it is increasingly recog...
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Article
Open AccessRobust adaptive optics for localization microscopy deep in complex tissue
Single-Molecule Localization Microscopy (SMLM) provides the ability to determine molecular organizations in cells at nanoscale resolution, but in complex biological tissues, where sample-induced aberrations ha...
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Article
Open AccessPhotoswitchable paclitaxel-based microtubule stabilisers allow optical control over the microtubule cytoskeleton
Small molecule inhibitors are prime reagents for studies in microtubule cytoskeleton research, being applicable across a range of biological models and not requiring genetic engineering. However, traditional c...
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Article
Taxanes convert regions of perturbed microtubule growth into rescue sites
Microtubules are polymers of tubulin dimers, and conformational transitions in the microtubule lattice drive microtubule dynamic instability and affect various aspects of microtubule function. The exact nature...
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Article
Open AccessProbing aggrephagy using chemically-induced protein aggregates
Selective types of autophagy mediate the clearance of specific cellular components and are essential to maintain cellular homeostasis. However, tools to directly induce and monitor such pathways are limited. H...
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Article
Open AccessAPC2 controls dendrite development by promoting microtubule dynamics
Mixed polarity microtubule organization is the signature characteristic of vertebrate dendrites. Oppositely oriented microtubules form the basis for selective cargo trafficking in neurons, however the mechanis...
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Protocol
Purification and Application of a Small Actin Probe for Single-Molecule Localization Microscopy
The cytoskeleton is involved in many cellular processes. Over the last decade, super-resolution microscopy has become widely available to image cytoskeletal structures, such as microtubules and actin, with gre...
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Article
DeActs: genetically encoded tools for perturbing the actin cytoskeleton in single cells
DeActs are genetically encoded tools that perturb the actin cytoskeleton. In contrast to drugs such as latrunculin, they can be targeted to specific cell types, which is demonstrated in the develo** mouse br...
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Article
Open AccessProbing cytoskeletal modulation of passive and active intracellular dynamics using nanobody-functionalized quantum dots
The cytoplasm is a highly complex and heterogeneous medium that is structured by the cytoskeleton. How local transport depends on the heterogeneous organization and dynamics of F-actin and microtubules is poor...
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Chapter
Microtubule Organization and Microtubule-Associated Proteins (MAPs)
Dendrites have a unique microtubule organization. In vertebrates, dendritic microtubules are organized in antiparallel bundles, oriented with their plus ends either pointing away or toward the soma. The mixed ...
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Article
Open AccessResolving bundled microtubules using anti-tubulin nanobodies
Microtubules are hollow biopolymers of 25-nm diameter and are key constituents of the cytoskeleton. In neurons, microtubules are organized differently between axons and dendrites, but their precise organizatio...
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Article
Optogenetic control of organelle transport and positioning
An optogenetic strategy allowing light-mediated recruitment of distinct cytoskeletal motor proteins to specific organelles is established; this technique enabled rapid and reversible activation or inhibition o...
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Protocol
Single Molecule Localization Microscopy to Study Neuronal Microtubule Organization
The highly complex and of is established by the , a of , such as and , and associated that provide and strength. In addition to providing structural , microtubules serve as tracks for long-...
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Article
Allosteric inhibition of kinesin-5 modulates its processive directional motility
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Article
The bipolar mitotic kinesin Eg5 moves on both microtubules that it crosslinks
During cell division, mitotic spindles are assembled by microtubule-based motor proteins1,2. The bipolar organization of spindles is essential for proper segregation of chromosomes, and requires plus-end-directed...
