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  1. No Access

    Article

    Egg white lysozyme purification with a chitin–silica-based affinity chromatographic matrix

    A composite biosorbent retaining non-covalently bound chitin in between layers of a silicon oxide matrix was assessed for lysozyme purification from undiluted egg white. The matrix can be shaped with big size ...

    Federico J. Wolman, Guillermo J. Copello… in European Food Research and Technology (2010)

  2. No Access

    Article

    Effect of various parameters on viability and growth of bacteria immobilized in sol–gel-derived silica matrices

    Immobilized bacteria are being extensively used for metabolite production, biocatalysts, and biosensor construction. However, long-term viability and metabolic activity of entrapped bacteria is affected by sev...

    Gisela S. Alvarez, María L. Foglia… in Applied Microbiology and Biotechnology (2009)

  3. No Access

    Article

    Immobilization of bacteria in silica matrices using citric acid in the sol–gel process

    The aim of this work was to use citric acid in the sol–gel process to generate an inorganic polymer that allows bacterial survival for long periods of time and to study the influence of different storage tempe...

    Gisela S. Alvarez, Martín F. Desimone… in Applied Microbiology and Biotechnology (2007)

  4. No Access

    Article

    Efficient preservation in a silicon oxide matrix of Escherichia coli, producer of recombinant proteins

    The aim of this work was to study the use of silicon oxide matrices for the immobilization and preservation of recombinant-protein-producing bacteria. We immobilized Escherichia coli BL21 transformants containing...

    Martín F. Desimone, Mauricio C. De Marzi… in Applied Microbiology and Biotechnology (2005)

  5. No Access

    Article

    Sol-gel immobilisation of Saccharomyces cerevisiae enhances viability in organic media

    The polyhydroxylated silane network of a sol-gel protected immobilised Saccharomyces cerevisiae against the effects of five organic solvents. The viability of immobilised yeast directly correlated with the logari...

    Martín F. Desimone, José Degrossi, Miguel D'Aquino, Luis E. Diaz in Biotechnology Letters (2003)

  6. No Access

    Article

    Ethanol tolerance in free and sol-gel immobilised Saccharomyces cerevisiae

    The tolerance of sol-gel immobilised and free Saccharomyces cerevisiae to ethanol was studied. The effects of ethanol preincubation time showed that the specific death velocity decreased from 2×105 c.f.u. min−1 f...

    Martín F. Desimone, José Degrossi, Miguel D'Aquino, Luis E. Diaz in Biotechnology Letters (2002)