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Article
Open AccessDevelopment of HPV16 mouse and dog models for more accurate prediction of human vaccine efficacy
Animal models are essential to understand the physiopathology of human diseases but also to evaluate new therapies. However, for several diseases there is no appropriate animal model, which complicates the dev...
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Protocol
CRISMERE Chromosome Engineering in Mouse and Rat
CRISPR/Cas9 technology is a versatile tool for engineering biology that has dramatically transformed our ability to manipulate genomes. In this protocol, we use its capacity to generate two double-strand break...
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Article
Open AccessAtp6ap2 ablation in adult mice impairs viability through multiple organ deficiencies
ATP6AP2 codes for the (pro)renin receptor and is an essential component of vacuolar H+ ATPase. Activating (pro)renin for conversion of Angiotensinogen to Angiotensin makes ATP6AP2 attract...
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Article
Open AccessEfficient and rapid generation of large genomic variants in rats and mice using CRISMERE
Modelling Down syndrome (DS) in mouse has been crucial for the understanding of the disease and the evaluation of therapeutic targets. Nevertheless, the modelling so far has been limited to the mouse and, even...
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Article
Open AccessAneuploidy screening of embryonic stem cell clones by metaphase karyoty** and droplet digital polymerase chain reaction
Karyotypic integrity is essential for the successful germline transmission of alleles mutated in embryonic stem (ES) cells. Classical methods for the identification of aneuploidy involve cytological analyses t...
