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Article
Open AccessMicroRNA 399 as a potential integrator of photo-response, phosphate homeostasis, and sucrose signaling under long day condition
Photoperiod-sensitivity is a critical endogenous regulatory mechanism for plant growth and development under specific environmental conditions, while phosphate and sucrose signaling processes play key roles in...
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Article
Overexpression of Zm-HINT1 Confers Salt and Drought Tolerance in Arabidopsis thaliana
Histidine triad nucleotide-binding protein 1 (HINT1) is highly conserved in many species and plays important roles in various biological processes. However, little is known about the responses of HINT1 to abio...
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Article
Long photoperiod affects the maize transition from vegetative to reproductive stages: a proteomic comparison between photoperiod-sensitive inbred line and its recurrent parent
Maize (Zea mays L.) is a typical short-day plant that is produced as an important food product and industrial material. The photoperiod is one of the most important evolutionary mechanisms enabling the adaptation...
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Article
Open AccessDual functions of the ZmCCT-associated quantitative trait locus in flowering and stress responses under long-day conditions
Photoperiodism refers to the ability of plants to measure day length to determine the season. This ability enables plants to coordinate internal biological activities with external changes to ensure normal gro...
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Article
Open AccessComparative proteomic analysis of the shoot apical meristem in maize between a ZmCCT-associated near-isogenic line and its recurrent parent
The ZmCCT, one of the most important genes affecting photoperiod response, delays flowering under long-day conditions in maize (Zea mays). In this study we used the isobaric tags for relative and absolute quantif...
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Article
Open AccessQuantitative analysis of changes in the phosphoproteome of maize induced by the plant hormone salicylic acid
Phytohormone salicylic acid (SA) plays an important role in regulating various physiological and biochemical processes. Our previous study identified several protein kinases responsive to SA, suggesting that p...
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Article
Open AccessQuantitative iTRAQ-based proteomic analysis of phosphoproteins and ABA-regulated phosphoproteins in maize leaves under osmotic stress
Abscisic acid (ABA) regulates various developmental processes and stress responses in plants. Protein phosphorylation/dephosphorylation is a central post-translational modification (PTM) in ABA signaling. Howe...
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Article
Overexpression of ZmMAPK1 enhances drought and heat stress in transgenic Arabidopsis thaliana
Mitogen-activated protein kinase (MAPK) signal transduction cascades play a crucial role in the response to extracellular stimuli in eukaryotes. A number of MAPK family genes have been isolated in plants, but ...
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Article
Overexpression of Zm-HINT1 in Arabidopsis thaliana enhances resistance to Fusarium graminearum
Histidine triad nucleotide binding protein 1(HINT1) plays an important role in many biological processes especially in cell biology, and they have been found in a wide variety of species. However, the function...
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Article
Phosphoproteomic analysis of the resistant and susceptible genotypes of maize infected with sugarcane mosaic virus
Protein phosphorylation plays a pivotal role in the regulation of many cellular events. No information is yet available, however, on protein phosphorylation in plants in response to virus infection. In this st...
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Article
Valid application of western blotting
Western blotting is a powerful and commonly used tool to identify and quantify a specific protein in a complex mixture. However, the systematic errors in the application of western blotting analysis are freque...
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Article
Identification and characterization of an E3 ubiquitin ligase Rbx1 in maize (Zea mays L.)
E3 ubiquitin ligases catalyze the ubiquitination of a variety of biologically significant protein substrates for targeted degradation through the 26S proteasome, as well as for nonproteolytic regulation of their ...
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Article
Comparative Proteomic Analysis of the Effects of Salicylic Acid and Abscisic Acid on Maize (Zea mays L.) Leaves
The phytohormones salicylic acid (SA) and abscisic acid (ABA) play essential roles in regulating plant growth, development, and stress responses. Using a proteomics-based approach, we compared the roles of SA ...
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Article
Molecular and functional characterization of ferritin in abalone Haliotis diversicolor supertexta
Ferritin is an iron storage protein that plays a key role in the processes of physiology and pathology. In the present study, the authors reported the ferritin gene from abalone Haliotis diversicolor supertexta, ...
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Article
Rickettsia-like organism infection associated with mass mortalities of blood clam, Tegillarca granosa, in the Yueqing Bay in China
A series of mass mortalities of the cultured blood clam, Tegillarca granosa, occurred in the Yueqing Bay of China from 2005 to 2009. An obligate intracellular prokaryote, designated as rickettsia-like organism (R...
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Article
Molecular cloning and expression analysis of an HINT1 homologue from maize (Zea mays L.)
Histidine triad nucleotide binding protein (HINT1) belongs to a histidine triad (HIT) superfamily, which contains a highly conserved His-X-His-X-His-XX motif (X is a hydrophobic amino acid) and plays an import...
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Article
Robust expression and association of ZmCCA1 with circadian rhythms in maize
In plants, the circadian clock is an endogenous mechanism that controls a wide range of biological processes. To date, as one of the key world crops, little is known about the molecular mechanism and component...