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Article
Open AccessDevelopment of pan-specific antibody against trimethyllysine for protein research
Trimethylation of the Nε-lysine residues in a protein is one of the most important events of posttranslational modifications. Simple methods for rapid detection and isolation of the Nε-trimethylated protein speci...
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Article
Rapid, in vivo, evaluation of antiangiogenic and antineoplastic gene products by nonviral transfection of tumor cells
Using a nonviral, electroporation-based gene transfection approach, we demonstrate the efficient and consistent transfection of two poorly immunogenic tumor cell lines: B16F10 melanoma and renal carcinoma (REN...
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Protocol
In Vitro and Ex Vivo Gene Delivery to Cells by Electroporation
Electroporation generally refers to the technique of permeabilizing cell membranes by applying a short and intense electric pulse across a cell, such that the barrier function of the membrane is instantaneousl...