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Article
Characterization of Neoschoengastia gallinarum from subtropical China by rDNA and identification of two genotypes based on mitochondrial cox1
Genetic variations in the 18S ribosomal DNA (18S), 28S ribosomal DNA (28S), second internal transcribed spacer of ribosomal DNA (ITS2), and mitochondrial cytochrome c oxidase subunit 1 (cox1) of Neoschoengastia g...
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Article
Open AccessGenome sequencing and analysis of fungus Hirsutella sinensis isolated from Ophiocordyceps sinensis
Ophiocordyceps sinensis has been used as a traditional medicine or healthy food in China for thousands of years. Hirsutella sinensis was reported as the only correct anamorph of O. sinensis. It is reported that t...
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Article
Investigation of antibiotic resistance determinants and virulence factors of uropathogenic Escherichia coli
Multidrug-resistant (MDR) uropathogenic Escherichia coli (UPEC) are prevalent throughout the world resulting in a major public health burden. In this research, we isolated and identified 28 MDR UPEC from one univ...
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Article
A new synthetic ligand that activates QscR and blocks antibiotic-tolerant biofilm formation in Pseudomonas aeruginosa
Quorum sensing (QS) has been recognized to play an important role in many pathogenic bacteria and has become a novel target for the treatment of infectious disease. Pseudomonas aeruginosa is highly resistant to a...
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Article
A new quorum-sensing inhibitor attenuates virulence and decreases antibiotic resistance in Pseudomonas aeruginosa
Quorum sensing (QS) has been a novel target for the treatment of infectious diseases. Here structural analogs of Pseudomonas aeruginosa autoinducer N-acyl homoserine lactone (AHL) were investigated for QS inhibit...
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Article
Enhancing catalytic activity of a hybrid xylanase through single substitution of Leu to Pro near the active site
A modified error-prone PCR and high-throughout screening system based on 96-well plate were employed to improve catalytic activity of a hybrid xylanase (ATx). The mutant (FSI-A124) with enhanced activity was f...
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Article
Selection of biochemical mutants of Aspergillus niger with enhanced extracellular ribonuclease production
Combined with u.v. irradiation and the nitrosoguanidine method, selection of biochemical mutants resistant to metabolic inhibitors (2-deoxy-D-glucose, antimycin A, sodium orthovanadate and sodium azide) was a ...
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Article
Screening and Mutagenesis of Aspergillus niger for the Improvement of Glucose-6-Phosphate Dehydrogenase Production
The Aspergillus niger strain ZBY-7 was selected as the original strain of glucose-6-phosphate dehydrogenase production. After mutagenesis of the strain by means of UV irradiation and nitrosoguanidine, mutants of
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Article
Optimization of glucose oxidase production by Aspergillus niger in a benchtop bioreactor using response surface methodology
Response surface methodology (RSM) was applied to optimize the speed of agitation and the rate of aeration for the maximum production of glucose oxidase (GOD) by Aspergillus niger. A 22 central composite design u...