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Article
A newly developed serum-free culture system: Clonal growth of human acute myelogeneous leukemia (AML) progenitors—a report of 14 AML cases
A new serum-free culture (SFC) system for human AML-CFU was established and the colony-promoting activity of four recombinant human hematopoietic growth factors (rhHGFs) including granulocyte-macrophage colony...
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Article
Detection of surface antigen expression of pre- and postcultured normal adult’s bone marrow cells by using ABC technique and its significance
The expression of surface antigens on both normal adult’s bone marrow and multipotential progenitor cells (CFU-Mix) was detected by use of ABC technique. The culture system of CFU-Mix was an ideal model for st...
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Article
Studies on human CFU-Mix microvolume culture by use of limiting dilution assay
By use of limiting dilution assay we investigated the bipotent and pluripotent hemopoietic progenitor cells (CFU-Mix) from 21 normal adults, cultured for 32 times. The CFU-Mix counts were 35.7 colonies/106 bone m...
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Article
Transmission electron microscopic observation of CFU-Mix culture in normal adults and leukemia patients
By use of transmission electron microscope we demonstrated that the limiting dilution assay is an important method for investigating differentiation of hemopoietic cells in vitro. If various growth factors wer...
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Article
Scanning electron microscopic observation of CFU-Mix of normal adults and leukemic patients including two cases of myeloid-lymphoid bipotent leukemia
We observed CFU-Mix of 4 normal adults and 11 cases of leukemia. The culture system used by us in this study represented a suitable model for studying differentiation of hemopoietic stem cells in vitro. The pa...
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Article
Studies on the relationship between leukemic fibroblast colony forming cell and hemopoietic cells
36 patients with leukemia, including AML, CML and ALL were studied. Fibroblast colony forming cells (CFU-F) in patients with different types of leukemia were less than in normal adults (P<0.05-0.001). There was n...
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Article
Research on normal human bone marrow CFU-GM by limiting dilution microculture
By using the newly developed technique of limiting dilution microculture we have succeeded in cultivating CFU-GM of human bone marrow. The clones, developed by this method, could be clearly and well identified...
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Article
Study on fibroblast colony forming units derived from bone marrow of normal adults and untreated patients with acute nonlymphocytic leukemia
The authors present a study of the growth of fibroblast colony forming units in tone marrow of normal adults and untreated patients with acute nonlymphocytic leukemia. The culture conditions and cytomorphology...
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Article
Prognostic significance of CFU-GM and karyotype study in 103 cases of untreated nonlymphocytic leukemia
The colony formation unit of granulo-monocytic progenitor cell (CFU-GM) growth pattern in the form of small clusters in 103 cases of untreated ANLL indicated favourable prognosis. These cases showed higher rat...
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Article
Correlation between growth pattern in vitro and karyotype of bone marrow cells and prognosis in adult acute nonlymphocytic leukemia
Growth pattern in vitro and karyotype of cultured bone marrow cells were studied in 31 patients with acute nonlymphocytic leukemia. Two different growth patterns were identified: cluster growth (pattern I), an...
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Article
Clinical manifestations, diagnosis and treatment of hypokalemic flabbiness disease
The most striking symptom of hypokalemic flabbiness disease is symmetric flaccid paralysis involving both the upper and lower extremities, which is characterized by weaker proximal muscle groups than distal on...
