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    Protocol

    Detection of TET-Oxidized 5-Methylcytosine Bases by Capillary Gel Electrophoresis

    The oxidation activity of the mammalian ten-eleven translocation dioxygenase (TET) on 5-methylcytosine (5mC) of DNA is usually monitored by analytical methods such as dot blotting and liquid chromatography-mas...

    Romualdas Vaisvila, Lise Hunault, Lana Saleh in DNA Modifications (2021)

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    Article

    Structure of a Naegleria Tet-like dioxygenase in complex with 5-methylcytosine DNA

    The Tet family of dioxygenase enzymes convert 5-methylcytosine to 5-hydroxymethylcytosine, 5-formylcytosine and 5-carboxylcytosine, which has an effect on gene expression; here the structure of NgTet1, a Tet-l...

    Hideharu Hashimoto, June E. Pais, **ng Zhang, Lana Saleh, Zheng-Qing Fu, Nan Dai in Nature (2014)

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    Article

    Characterization of NO adducts of the diiron center in protein R2 of Escherichia coli ribonucleotide reductase and site-directed variants; implications for the O2 activation mechanism*

    The R2 subunit of Escherichia coli ribonucleotide reductase contains a diiron site that reacts with O2 to produce a tyrosine radical (Y122·). In wild-type R2 (R2-wt), the first observable reaction intermediate is...

    Shen Lu, Eduardo Libby, Lana Saleh in JBIC Journal of Biological Inorganic Chemi… (2004)