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Protocol
Detection of TET-Oxidized 5-Methylcytosine Bases by Capillary Gel Electrophoresis
The oxidation activity of the mammalian ten-eleven translocation dioxygenase (TET) on 5-methylcytosine (5mC) of DNA is usually monitored by analytical methods such as dot blotting and liquid chromatography-mas...
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Article
Structure of a Naegleria Tet-like dioxygenase in complex with 5-methylcytosine DNA
The Tet family of dioxygenase enzymes convert 5-methylcytosine to 5-hydroxymethylcytosine, 5-formylcytosine and 5-carboxylcytosine, which has an effect on gene expression; here the structure of NgTet1, a Tet-l...
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Article
Characterization of NO adducts of the diiron center in protein R2 of Escherichia coli ribonucleotide reductase and site-directed variants; implications for the O2 activation mechanism*
The R2 subunit of Escherichia coli ribonucleotide reductase contains a diiron site that reacts with O2 to produce a tyrosine radical (Y122·). In wild-type R2 (R2-wt), the first observable reaction intermediate is...