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Article
Open AccessPerspectives for biocatalytic lignin utilization: cleaving 4-O-5 and Cα–Cβ bonds in dimeric lignin model compounds catalyzed by a promiscuous activity of tyrosinase
In the biorefinery utilizing lignocellulosic biomasses, lignin decomposition to value-added phenolic derivatives is a key issue, and recently biocatalytic delignification is emerging owing to its superior sele...
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Article
Open AccessExpression and characterization of Pantoea CO dehydrogenase to utilize CO-containing industrial waste gas for expanding the versatility of CO dehydrogenase
Although aerobic CO dehydrogenases (CODHs) might be applicable in various fields, their practical applications have been hampered by low activity and no heterologous expression. We, for the first time, could f...
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Article
Highly sensitive and selective dopamine detection by an amperometric biosensor based on tyrosinase/MWNT/GCE
Dopamine (3,4-dihydroxylphenyl ethylamine) is the most significant neurotransmitter in the human nervous system. Abnormal dopamine levels cause fatal neurological disorders, and thus measuring dopamine level i...
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Article
Selective determination of dopamine with an amperometric biosensor using electrochemically pretreated and activated carbon/tyrosinase/Nafion®-modified glassy carbon electrode
Dopamine, the most important neurotransmitter in the human brain, controls various functions. Dopamine deficiency causes fatal neurological disorders such as Parkinson’s disease. Even though various types of e...
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Article
Open AccessA dye-decolorizing peroxidase from Bacillus subtilis exhibiting substrate-dependent optimum temperature for dyes and β-ether lignin dimer
In the biorefinery using lignocellulosic biomass as feedstock, pretreatment to breakdown or loosen lignin is important step and various approaches have been conducted. For biological pretreatment, we screened Bac...
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Article
Overview on the biotechnological production of l-DOPA
l-DOPA (3,4-dihydroxyphenyl-l-alanine) has been widely used as a drug for Parkinson’s disease caused by deficiency of the neurotransmitter dopamine. Since Monsanto developed the commercia...
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Article
Recent progress in nanobiocatalysis for enzyme immobilization and its application
Recent advances in nanotechnology have provided various nanoscale materials that can be used as support for enzyme immobilization. Nanobiocatalysis integrating the biocatalyst and nanoscale materials is drawin...
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Article
Conversion of levulinic acid to 2-butanone by acetoacetate decarboxylase from Clostridium acetobutylicum
In this study, a novel system for synthesis of 2-butanone from levulinic acid (γ-keto-acid) via an enzymatic reaction was developed. Acetoacetate decarboxylase (AADC; E.C. 4.1.1.4) from Clostridium acetobutylicum
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Article
Screening of stable cutinase from Fusarium solani pisi using plasmid display system
Although enzymes are potential candidates for industrial catalysts, their industrial applications have been limited because they are easily deactivated under harsh operational conditions. In this study, a plas...
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Article
Kinetics of glycerol effect on biodiesel production for optimal feeding of methanol
The enzymatic production of biodiesel has been considered as an eco-friendly process. Candida antarctica lipase B (CalB) has been studied for its application for biodiesel production because of its high activity ...
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Article
Development of an amphiphilic matrix for immobilization of Candida antartica lipase B for biodiesel production
Biodiesel has been greatly interested as an alternative fuel and is produced by a transesterification reaction of oil with alcohol. Recently, microbial lipases have been used for biodiesel production. Among th...
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Article
Mediator-free glucose/O2 biofuel cell based on a 3-dimensional glucose oxidase/SWNT/polypyrrole composite electrode
In this study, a mediator-free glucose/O2 bio-fuel cell was developed based on a 3-dimensional carbon nanomaterial/polypyrrole composite with glucose oxidase and tyrosinase as the anodic and cathodic catalysts, r...
