-
Article
Open AccessThe use of NADH anisotropy to investigate mitochondrial cristae alignment
Life may be expressed as the flow of electrons, protons, and other ions, resulting in large potential difference. It is also highly photo-sensitive, as a large proportion of the redox capable molecules it reli...
-
Article
Open AccessStructural basis for the modulation of MRP2 activity by phosphorylation and drugs
Multidrug resistance-associated protein 2 (MRP2/ABCC2) is a polyspecific efflux transporter of organic anions expressed in hepatocyte canalicular membranes. MRP2 dysfunction, in Dubin-Johnson syndrome or by of...
-
Article
Open AccessExperimental phasing opportunities for macromolecular crystallography at very long wavelengths
Despite recent advances in cryo-electron microscopy and artificial intelligence-based model predictions, a significant fraction of structure determinations by macromolecular crystallography still requires expe...
-
Article
Open AccessAuthor Correction: OmpK36-mediated Carbapenem resistance attenuates ST258 Klebsiella pneumoniae in vivo
-
Article
Open AccessMating pair stabilization mediates bacterial conjugation species specificity
Bacterial conjugation mediates contact-dependent transfer of DNA from donor to recipient bacteria, thus facilitating the spread of virulence and resistance plasmids. Here we describe how variants of the plasmi...
-
Protocol
PELDOR/DEER: An Electron Paramagnetic Resonance Method to Study Membrane Proteins in Lipid Bilayers
Every membrane protein is involved in close interactions with the lipid environment of cellular membranes. The annular lipids, that are in direct contact with the polypeptide, can in principle be seen as an in...
-
Article
Open AccessOmpK36-mediated Carbapenem resistance attenuates ST258 Klebsiella pneumoniae in vivo
Carbapenem-resistance in Klebsiella pneumoniae (KP) sequence type ST258 is mediated by carbapenemases (e.g. KPC-2) and loss or modification of the major non-selective porins OmpK35 and OmpK36. However, the mechan...
-
Article
Open AccessCrystal structure and epitope analysis of house dust mite allergen Der f 21
Group 21 and 5 allergens are homologous house dust mite proteins known as mid-tier allergens. To reveal the biological function of group 21 allergens and to understand better the allergenicity of the rDer f 21...
-
Article
Open AccessSolid immersion microscopy images cells under cryogenic conditions with 12 nm resolution
Super-resolution fluorescence microscopy plays a crucial role in our understanding of cell structure and function by reporting cellular ultrastructure with 20–30 nm resolution. However, this resolution is insu...
-
Article
Open AccessSubstrate-bound outward-open structure of a Na+-coupled sialic acid symporter reveals a new Na+ site
Many pathogenic bacteria utilise sialic acids as an energy source or use them as an external coating to evade immune detection. As such, bacteria that colonise sialylated environments deploy specific transport...
-
Article
Structural basis for hijacking siderophore receptors by antimicrobial lasso peptides
Lasso peptides are antimicrobial bacterial peptides that hijack outer membrane siderophores to kill target cells. The structural and biochemical basis of antimicrobial lasso peptide MccJ25 binding to sideropho...
-
Article
Wza the translocon for E. coli capsular polysaccharides defines a new class of membrane protein
Many bacteria possess an external polysaccharide 'capsule' that helps them evade the host immune system and colonize surfaces. As a point of contact between host and pathogen, the capsule is a potential target...
