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    Article

    Chemical Groups that Adhere to the Surfaces of Living Malignant Cells

    We determined the adhesion of particles with phenyl, carboxylic acid (COOH), amine, dialkyl phosphonate, ester, and hydroxyl groups to malignant and nonmalignant cells, in order to better design drug delivery sys...

    Cathy E. McNamee, Yuki Aso, Shinpei Yamamoto, Yoshinobu Fukumori in Pharmaceutical Research (2007)

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    Article

    Nanoscale Indentation of Polymer and Composite Particles by Atomic Force Microscopy

    Atomic Force Microscopy (AFM) was employed to probe the mechanical properties of surface-charged polymethylmethacrylate (PMMA)-based terpolymer and a composite terpolymer core-silica shell nanosphere in air an...

    Silvia Armini, Ivan U. Vakarelski, Caroline M. Whelan in MRS Online Proceedings Library (2006)

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    Article

    Sublimation Phenomena in Slit Nanopores: Lennard-Jones Phase Diagram

    Sublimation phenomena of Lennard-Jones fluids in slit-shaped nanopores were examined. Vapor-phase pressure at which a solid phase forms in a pore at a constant temperature is determined by the authors’ molecul...

    Hideki Kanda, Minoru Miyahara, Ko Higashitani in Adsorption (2005)

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    Article

    Formation of ots self-assembled monolayer on glass surface investigated by AFM

    The whole process of octadecyltrichlorosilane (OTS) self-assembled monolayer formation on glass surface was clearly revealed by using Atomic Force Microscope (AFM). The growth regularity pattern of OTS self-as...

    Xu Guo-hua, Ko Higashitani in Journal of Zhejiang University-SCIENCE A (2000)

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    Article

    Development and application of thermo-sensitive magnetic immunomicrospheres for antibody purification

    Ultrafine magnetite particles were prepared by a co-precipitation method. The poly-(styrene/N-isopropylacrylamide/methacrylic acid) latex particles containing ultrafine magnetite [magnetic P(St/NIPAM/MAA)] were p...

    Akihiko Kondo, Hiroko Kamura, Ko Higashitani in Applied Microbiology and Biotechnology (1994)

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    Article

    Purification of fusion proteins using affinity microspheres in aqueous two-phase systems

    Affinity microspheres were prepared by immobilizing human γ-globulin (HγGb) onto carboxylated poly (styrene/acrylamide) latex particles [P(St/AAm)-H; average diameter 0.33 μm], which were prepared by emulsifie...

    Akihiko Kondo, Tetsuya Kaneko, Ko Higashitani in Applied Microbiology and Biotechnology (1993)

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    Article

    Kinetic and circular dichroism studies of enzymes adsorbed on ultrafine silica particles

    Negatively charged ultrafine silica particles (average diameter 20 nm) were used as support materials for adsorption immobilization of porcine trypsin, horseradish peroxidase, and bovine catalase under various...

    Akihiko Kondo, Fumiyasu Murakami, Masako Kawagoe in Applied Microbiology and Biotechnology (1993)

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    Chapter and Conference Paper

    Affinity Purification of Proteins Using Colloidal Particles

    Application of colloidal particles to bioseparations has found to be of interest for large scale purification [1]. To develop a novel affinity separation process based on colloidal particles, it is necessary t...

    Akihiko Kondo, Katsuhiro Yoshioka, Ryo Yamasaki in Biochemical Engineering for 2001 (1992)