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Article
Chemical Groups that Adhere to the Surfaces of Living Malignant Cells
We determined the adhesion of particles with phenyl, carboxylic acid (COOH), amine, dialkyl phosphonate, ester, and hydroxyl groups to malignant and nonmalignant cells, in order to better design drug delivery sys...
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Article
Nanoscale Indentation of Polymer and Composite Particles by Atomic Force Microscopy
Atomic Force Microscopy (AFM) was employed to probe the mechanical properties of surface-charged polymethylmethacrylate (PMMA)-based terpolymer and a composite terpolymer core-silica shell nanosphere in air an...
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Article
Sublimation Phenomena in Slit Nanopores: Lennard-Jones Phase Diagram
Sublimation phenomena of Lennard-Jones fluids in slit-shaped nanopores were examined. Vapor-phase pressure at which a solid phase forms in a pore at a constant temperature is determined by the authors’ molecul...
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Article
Formation of ots self-assembled monolayer on glass surface investigated by AFM
The whole process of octadecyltrichlorosilane (OTS) self-assembled monolayer formation on glass surface was clearly revealed by using Atomic Force Microscope (AFM). The growth regularity pattern of OTS self-as...
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Article
Development and application of thermo-sensitive magnetic immunomicrospheres for antibody purification
Ultrafine magnetite particles were prepared by a co-precipitation method. The poly-(styrene/N-isopropylacrylamide/methacrylic acid) latex particles containing ultrafine magnetite [magnetic P(St/NIPAM/MAA)] were p...
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Article
Purification of fusion proteins using affinity microspheres in aqueous two-phase systems
Affinity microspheres were prepared by immobilizing human γ-globulin (HγGb) onto carboxylated poly (styrene/acrylamide) latex particles [P(St/AAm)-H; average diameter 0.33 μm], which were prepared by emulsifie...
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Article
Kinetic and circular dichroism studies of enzymes adsorbed on ultrafine silica particles
Negatively charged ultrafine silica particles (average diameter 20 nm) were used as support materials for adsorption immobilization of porcine trypsin, horseradish peroxidase, and bovine catalase under various...
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Chapter and Conference Paper
Affinity Purification of Proteins Using Colloidal Particles
Application of colloidal particles to bioseparations has found to be of interest for large scale purification [1]. To develop a novel affinity separation process based on colloidal particles, it is necessary t...