8 Result(s)
within
Klaas J. Hellingwerf
English
Plant Breeding/Biotechnology
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Article
Open AccessUsing osmotic stress to stabilize mannitol production in Synechocystis sp. PCC6803
Mannitol is a C(6) polyol that is used in the food and medical sector as a sweetener and antioxidant, respectively. The sustainable production of mannitol, especially via the direct conversion of CO2 by photosynt...
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Article
Open AccessAlignment of microbial fitness with engineered product formation: obligatory coupling between acetate production and photoautotrophic growth
Microbial bioengineering has the potential to become a key contributor to the future development of human society by providing sustainable, novel, and cost-effective production pipelines. However, the sustaine...
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Article
Open AccessSingle-cell screening of photosynthetic growth and lactate production by cyanobacteria
Photosynthetic cyanobacteria are attractive for a range of biotechnological applications including biofuel production. However, due to slow growth, screening of mutant libraries using microtiter plates is not ...
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Article
Open AccessQuantitative proteomics analysis of an ethanol- and a lactate-producing mutant strain of Synechocystis sp. PCC6803
This study aimed at exploring the molecular physiological consequences of a major redirection of carbon flow in so-called cyanobacterial cell factories: quantitative whole-cell proteomics analyses were carried...
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Article
Open AccessExploring metabolic engineering design principles for the photosynthetic production of lactic acid by Synechocystis sp. PCC6803
Molecular engineering of the intermediary physiology of cyanobacteria has become important for the sustainable production of biofuels and commodity compounds from CO2 and sunlight by “designer microbes.” The chem...
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Chapter
The Electrochemical Proton Gradient and Solute Transport in Rhodopseudo-Monas Sphaeroides
Rhodopseudomonas sphaeroides is a Gram-negative phototrophic bacterium which can grow either aerobically in the dark or anaerobically in the light. The bacteria are surrounded by a cell-envelope ...
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Chapter
Reaction Centers from Rhodopseudomonas Sphaeroides in Reconstituted Phospholipid Vesicles. Structural Properties and Light-Dependent Proton Translocation
Reaction Centers (RC’s) from phototrophic bacteria catalyze light-driven transmembrane electron transfer as a first step in the (cyclic) electron transfer chain of such bacteria (for a review see: Okamura et a...
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Chapter
The Lactose Carrier of Escherichia Coli Functionally Incorporated in Rhodopseudomonas Sphaeroides Obeys the Regulatory Conditions of the Phototrophic Bacterium
Regulation of solute transport in Rps. sphaeroides is such that the presence of a proton motive force by itself is not enough for solute transport to take place. In addition linear or cyclic electron transfer is ...
