Abstract
Regulation of solute transport in Rps. sphaeroides is such that the presence of a proton motive force by itself is not enough for solute transport to take place. In addition linear or cyclic electron transfer is required. In cells incubated anaerobically in the dark, no uptake of alanine is measurable, in spite of the presence of a high proton motive force. Illumination of such a suspension initiates cyclic electron transfer and allows solute uptake to proceed. The uptake rate of alanine increases with the light intensity (Elferink et al., 1983).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Elferink MGL, Friedberg I, Hellingwerf KJ and Konings WN (1983) The role of the proton motive force and electron flow in light-driven solute transport in Rhodopseudomonas sphaeroides, Eur.J.Biochem. 129, 583–587.
Forster DL, Garcia ML, Newman ML, Patel L and Kaback HR (1982) Lactoseproton symport by purified lac carrier protein, Biochem. 21, 3634–3638.
Ghazi A and Shechter E (1981) Lactose transport in E. coli cells. Dependence of kinetic parameters on the transmembrane electrical potential difference, Biochim.Biophys.Acta 645, 305–315.
Konings WN and Robillard GT (1982) Physical mechanism for regulation of proton solute symport in Escherichia coli, Proc.Natl.Acad.Sci. USA 79, 5480–5484.
Nicolay K, Lolkema JS, Hellingwerf KJ, Kaptein R and Konings WN (1981) Quantitative agreement between the values for the light-induced ∆pH in Rhodopseudomonas sphaeroides measured with automated flow-dialysis and 31P NMR, Febs Lett. 123, 319–323.
Robertson DE, Kaszorowski GJ, Garcia M and Kaback HR (1980) Active transport in membrane vesicles from Escherichia coli: The electrochemical proton gradient alters the distribution of the lac carrier between two different kinetic states, Biochem. 19, 5692–5702.
Schuldiner S and Kaback HR (1975) Membrane potential and active transport in membrane vesicles from Escherichia coli, Biochem. 14, 5451–5461.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1984 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Elferink, M.G.L., Hellingwerf, K.J., Konings, W.N., Kaplan, S., Nano, F.E. (1984). The Lactose Carrier of Escherichia Coli Functionally Incorporated in Rhodopseudomonas Sphaeroides Obeys the Regulatory Conditions of the Phototrophic Bacterium. In: Sybesma, C. (eds) Advances in Photosynthesis Research. Advances in Agricultural Biotechnology, vol 2. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-6368-4_106
Download citation
DOI: https://doi.org/10.1007/978-94-017-6368-4_106
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-247-2943-2
Online ISBN: 978-94-017-6368-4
eBook Packages: Springer Book Archive