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Article
Open AccessProcessing of a single ribonucleotide embedded into DNA by human nucleotide excision repair and DNA polymerase η
DNA polymerases often incorporate non-canonical nucleotide, i.e., ribonucleoside triphosphates into the genomic DNA. Aberrant accumulation of ribonucleotides in the genome causes various cellular abnormalities...
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Article
Open AccessConstruction and applications of exon-trap** gene-targeting vectors with a novel strategy for negative selection
Targeted gene modification by homologous recombination provides a powerful tool for studying gene function in cells and animals. In higher eukaryotes, non-homologous integration of targeting vectors occurs sev...
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Article
Open AccessNap1 stimulates homologous recombination by RAD51 and RAD54 in higher-ordered chromatin containing histone H1
Homologous recombination plays essential roles in mitotic DNA double strand break (DSB) repair and meiotic genetic recombination. In eukaryotes, RAD51 promotes the central homologous-pairing step during homolo...
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Article
Histone methyltransferases: regulation of transcription and contribution to human disease
Histone modifications contribute to the precise regulation of transcription by recruiting non-histone proteins and controlling chromatin conformation. These covalent modifications are dynamically regulated by ...
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Article
A histone H3 lysine 36 trimethyltransferase links Nkx2-5 to Wolf–Hirschhorn syndrome
Histone H3 trimethylation at lysine 36 (H3K36me3) is associated with actively transcribed regions and has been proposed to provide landmarks for ongoing transcription. Here, an H3K36me3-specific histone methyl...
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Protocol
Reconstitution of Chromatin In Vitro
It is now generally believed that DNA methylation is responsible for genomic imprinting in mammals (1). Recent experimental evidence has provided an elegant mechanism for repression of gene expression by DNA meth...
