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  1. Article

    Open Access

    Gender identification of the horsehair crab, Erimacrus isenbeckii (Brandt, 1848), by image recognition with a deep neural network

    Appearance-based gender identification of the horsehair crab [Erimacrus isenbeckii (Brandt, 1848)] is important for preventing indiscriminate fishing of female crabs. Although their gender is easily identified by...

    Yoshitaka Ueki, Kenji Toyota, Tsuyoshi Ohira, Ken Takeuchi in Scientific Reports (2023)

  2. Article

    Open Access

    The role of mesenchymal estrogen receptor 1 in mouse uterus in response to estrogen

    Estrogens play important roles in uterine growth and homeostasis through estrogen receptors (ESR1 and ESR2). To address the role of ESR1-mediated tissue events in the murine uterus, we analyzed mice with a mes...

    Keita Furuminato, Saki Minatoya, Eriko Senoo, Tatsuki Goto in Scientific Reports (2023)

  3. Article

    Open Access

    Kynurenine promotes Calcitonin secretion and reduces cortisol in the Japanese flounder Paralichthys olivaceus

    Deep ocean water (DOW) exerts positive effects on the growth of marine organisms, suggesting the presence of unknown component(s) that facilitate their aquaculture. We observed that DOW suppressed plasma corti...

    Takahiro Ikari, Yukihiro Furusawa, Yoshiaki Tabuchi, Yusuke Maruyama in Scientific Reports (2023)

  4. Article

    Open Access

    Deep ocean water alters the cholesterol and mineral metabolism of squid Todarodes pacificus and suppresses its weight loss

    This study is the first to demonstrate that deep ocean water (DOW) has physiological significant effects on squid. After 36 h of rearing squids, those reared with DOW had significantly higher total and free ch...

    Kaito Hatano, Masa-Aki Yoshida, Jun Hirayama, Yoichiro Kitani in Scientific Reports (2023)

  5. Article

    Open Access

    Eyestalk transcriptome and methyl farnesoate titers provide insight into the physiological changes in the male snow crab, Chionoecetes opilio, after its terminal molt

    The snow crab, Chionoecetes opilio, is a giant deep-sea brachyuran. While several decapod crustaceans generally continue to molt and grow throughout their lifetime, the snow crab has a fixed number of molts. Adol...

    Kenji Toyota, Takeo Yamamoto, Tomoko Mori, Miyuki Mekuchi in Scientific Reports (2023)

  6. Article

    Open Access

    Adaptation to the shallow sea floor environment of a species of marine worms, Oligobrachia mashikoi, generally inhabiting deep-sea water

    Beard worms from the family Siboglinidae, are peculiar animals and are known for their symbiotic relationships with sulfur bacteria. Most Siboglinids inhabit the deep-sea floor, thus making difficult to make a...

    Shouzo Ogiso, Kazuki Watanabe, Yusuke Maruyama, Hiroshi Miyake in Scientific Reports (2023)

  7. No Access

    Article

    Annual reproductive cycle of the greasyback shrimp Metapenaeus ensis in Ise Bay, Japan

    The greasyback shrimp Metapenaeus ensis is widely distributed along the coast of India and the West Pacific where it is an important fisheries species. We have examined seasonal changes in ovarian development, sp...

    Fumihiro Yamane, Katsuyoshi Suitoh, Takuji Okumura, Kenji Toyota in Fisheries Science (2022)

  8. Article

    Open Access

    Transgenerational response to early spring warming in Daphnia

    Temperature and photoperiod regulate key fitness traits in plants and animals. However, with temperature increase due to global warming, temperature cue thresholds are experienced at shorter photoperiods, disr...

    Kenji Toyota, Maria Cambronero Cuenca, Vignesh Dhandapani in Scientific Reports (2019)

  9. No Access

    Chapter

    Environmental Control of Sex Differentiation in Daphnia

    The cladoceran crustacean genus Daphnia (daphnids) exhibits a unique reproductive strategy in response to changes in environmental conditions of their habitat. This is known as environmental sex determination (ES...

    Kenji Toyota, Norihisa Tatarazako in Reproductive and Developmental Strategies (2018)

  10. Article

    Open Access

    Metabolomics reveals an involvement of pantothenate for male production responding to the short-day stimulus in the water flea, Daphnia pulex

    Under favorable conditions, the micro-crustacean Daphnia pulex produces female offspring by parthenogenesis, whereas under unfavorable conditions, they produce male offspring to induce sexual reproduction (enviro...

    Kenji Toyota, Alex Gavin, Shinichi Miyagawa, Mark R. Viant in Scientific Reports (2016)

  11. Article

    Open Access

    NMDA receptor activation upstream of methyl farnesoate signaling for short day-induced male offspring production in the water flea, Daphnia pulex

    The cladoceran crustacean Daphnia pulex produces female offspring by parthenogenesis under favorable conditions, but in response to various unfavorable external stimuli, it produces male offspring (environmental ...

    Kenji Toyota, Hitoshi Miyakawa, Katsushi Yamaguchi, Shuji Shigenobu in BMC Genomics (2015)

  12. Article

    Open Access

    Targeted gene disruption by use of transcription activator-like effector nuclease (TALEN) in the water flea Daphnia pulex

    The cosmopolitan microcrustacean Daphnia pulex provides a model system for both human health research and monitoring ecosystem integrity. It is the first crustacean to have its complete genome sequenced, an unpre...

    Chizue Hiruta, Yukiko Ogino, Tetsushi Sakuma, Kenji Toyota in BMC Biotechnology (2014)

  13. Article

    Open Access

    Roles of ecdysteroids for progression of reproductive cycle in the fresh water crustacean Daphnia magna

    Daphnia magna exhibits a parthenogenetic reproductive cycle linked to a moulting cycle, but regulatory mechanisms of neither moulting nor reproductive cycle are understood in daphnids. Moulting is regulated by ec...

    Eri Sumiya, Yukiko Ogino, Hitoshi Miyakawa, Chizue Hiruta in Frontiers in Zoology (2014)

  14. Article

    Open Access

    Development of a microinjection system for RNA interference in the water flea Daphnia pulex

    The ubiquitous, freshwater microcrustacean Daphnia pulex provides a model system for both human health research and monitoring ecosystem integrity. It is the first crustacean to have a well annotated, reference g...

    Chizue Hiruta, Kenji Toyota, Hitoshi Miyakawa, Yukiko Ogino in BMC Biotechnology (2013)

  15. No Access

    Article

    A mutation in the receptor Methoprene-tolerant alters juvenile hormone response in insects and crustaceans

    Juvenile hormone is an essential regulator of major developmental and life history events in arthropods. Most of the insects use juvenile hormone III as the innate juvenile hormone ligand. By contrast, crustac...

    Hitoshi Miyakawa, Kenji Toyota, Ikumi Hirakawa, Yukiko Ogino in Nature Communications (2013)

  16. Article

    Open Access

    Molecular cloning of doublesex genes of four cladocera (water flea) species

    The gene doublesex (dsx) is known as a key factor regulating genetic sex determination in many organisms. We previously identified two dsx genes (DapmaDsx1 and DapmaDsx2) from a freshwater branchiopod crustacean,...

    Kenji Toyota, Yasuhiko Kato, Masaru Sato, Naomi Sugiura, Shinichi Miyagawa in BMC Genomics (2013)