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Article
Pollution Control of Nitrate-selective Membrane by the Inner Solution and On-site Monitoring of Nitrate Concentration in Soil
A liquid-membrane type nitrate-selective electrode was improved to lower the influence of contaminants by modifying its inner electrode system from Ag | AgCl | Cl− to Ag | Ag+. The NO3−-selective electrode displa...
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Article
Automatic Management of Nutrient Solution for Hydroponics—Construction of Multi-ion Stat—
In order to improve plant factories, an appropriate control system on fertilization is urgently required. An automatic management system to control nutrient concentration was constructed using a programmable l...
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Article
Fabrication of a Phosphate Ion Selective Electrode Based on Modified Molybdenum Metal
A phosphate ion-selective electrode using molybdenum metal was constructed. The modified molybdenum electrode responded to HPO42- in the presence of molybdenum dioxide and molybdophosphate (PMo12O403-) on the sur...
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Chapter
Bioelectrochemical and Reversible Interconversion in the Proton/Hydrogen and Carbon Dioxide/Formate Redox Systems and Its Significance in Future Energy Systems
Bioelectrocatalytic reactions based on hydrogenase (H2ase) and molybdopterin fructose dehydrogenase (FDH) realize electrochemically reversible redox reactions of the 2H+/H2 and CO2/HCOO− redox couples, respective...
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Article
Open AccessThe influence of the shape of Au nanoparticles on the catalytic current of fructose dehydrogenase
Graphite electrodes were modified with triangular (AuNTrs) or spherical (AuNPs) nanoparticles and further modified with fructose dehydrogenase (FDH). The present study reports the effect of the shape of these ...
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Article
Construction of an Automatic Nutrient Solution Management System for Hydroponics-Adjustment of the K+-Concentration and Volume of Water
An automatic management system for nutrient solutions was constructed using a programmable logic controller (PLC) and a K+-ion selective electrode (K+-ISE). The concentration of K+ was monitored by the K+-ISE. Wh...
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Article
Construction of Nitrate-selective Electrodes and Monitoring of Nitrates in Hydroponic Solutions
A liquid-membrane type nitrate-selective electrode was constructed, in which the responding membrane contained polyvinylchloride, o-nitrophenyloctylether and tetraheptylammonium nitrate. The NO3−-selective electr...
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Article
Nanostructured Porous Electrodes by the Anodization of Gold for an Application as Scaffolds in Direct-electron-transfer-type Bioelectrocatalysis
In this study, nanostructured porous gold electrodes were prepared by the anodization of gold in the presence of oxalic acid or glucose as a reductant, and applied as scaffolds for direct electron transfer (DE...
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Article
Simultaneous Detection of Lactate Enantiomers Based on Diffusion-controlled Bioelectrocatalysis
Amperometric biosensors were constructed for the simultaneous detection of lactate enantiomers. The enantioselectivity of the sensor is based on NAD-dependent L- and D-lactate dehydrogenases that, respectively...
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Article
Electrochemical Study on the Extracellular Electron Transfer Pathway from Shewanella Strain Hac319 to Electrodes
Shewanella can transfer electrons to various extracellular electron acceptors. We electrochemically investigated the pathway of extracellular electron transfer from Shewanella strain Hac319 to electrodes. A resti...
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Article
Open AccessThe influence of pH and divalent/monovalent cations on the internal electron transfer (IET), enzymatic activity, and structure of fructose dehydrogenase
We report on the influence of pH and monovalent/divalent cations on the catalytic current response, internal electron transfer (IET), and structure of fructose dehydrogenase (FDH) by using amperometry, spectro...
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Article
Direct Electron Transfer-type Bioelectrocatalysis of Peroxidase at Mesoporous Carbon Electrodes and Its Application for Glucose Determination Based on Bienzyme System
Non-catalytic direct electron transfer (DET) signal of Compound I of horseradish peroxidase (POD) was first detected at 0.7 V on POD/carbon nanotube mixture-modified electrodes. Excellent performance of DET-ty...
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Article
Diffusion-controlled Mediated Electron Transfer-type Bioelectrocatalysis Using Microband Electrodes as Ultimate Amperometric Glucose Sensors
We performed numerical simulations on an extremely fast, mediated, electron transfer-type bioelectrocatalytic reaction using a microband electrode. The simulations under fast-enzyme-kinetics conditions predict...
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Article
Analysis of Ion Transport through a Single Channel of Gramicidin A in Bilayer Lipid Membranes
Ion transport through a single channel of gramicidin A (GA) within the bilayer lipid membrane (BLM) between two aqueous phases (W1 and W2) has been analyzed based on the electroneutrality principle. The single...
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Article
Factors to Govern Soluble and Insoluble Aggregate-formation in Monoclonal Antibodies
The aggregation formation of monoclonal antibodies as biopharmaceuticals induced by heat stress was evaluated by size-exclusion chromatography, and the formation rate was correlated with several physicochemica...
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Article
Fabrication and Characterization of Ultrathin-ring Electrodes for Pseudo-steady-state Amperometric Detection
The fabrication of ultrathin-ring electrodes with a diameter of 2 mm and a thickness of 100 nm is established. The ultrathin-ring electrodes provide a large density of pseudo-steady-state currents, and realize...
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Article
Influence of the Circulating Current on the Propagation of the Change in Membrane Potential
The propagation of the change in potential differences across liquid membranes from the potential-sending cell to the potential-receiving cell was investigated by use of a system combined with three liquid mem...
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Article
Separation and quantification of monoclonal-antibody aggregates by hollow-fiber-flow field-flow fractionation
Hollow-fiber-flow field-flow fractionation (HF5) separates protein molecules on the basis of the difference in the diffusion coefficient, and can evaluate the aggregation ratio of proteins. However, HF5 is sti...
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Article
Diffusion-controlled Detection of Glucose with Microelectrodes in Mediated Bioelectrocatalytic Oxidation
This paper describes a diffusion-controlled electrolysis of glucose with mediated bioelectrocatalysis at microdisk-electrodes. Under conditions of an extremely fast enzyme reaction, compared with the diffusion...
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Article
Effect of Counter Ions on the Transport Current Across Membranes Containing KAT1 Potassium Channel
Ion transports from one aqueous phase (W1) to another (W2) across bilayer lipid membranes (BLM) containing KAT1 potassium channel were investigated by recording current fluctuations. KAT1 channel is a voltage-...
