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Article
Open AccessBioanalytical technologies using temperature-responsive polymers
In recent decades, various bioanalytical technologies have been investigated for appropriate medical treatment and effective therapy. Temperature-responsive chromatography is a promising bioanalytical technolo...
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Article
Open AccessUmbilical cord-derived mesenchymal stem cell sheets transplanted subcutaneously enhance cell retention and survival more than dissociated stem cell injections
Human umbilical cord-derived mesenchymal stem cell (hUC-MSC) sheets have recently attracted attention as an alternative approach to injected cell suspensions for stem cell therapy. However, cell engraftment an...
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Article
Open AccessChromatography columns packed with thermoresponsive-cationic-polymer-modified beads for therapeutic drug monitoring
Therapeutic drug monitoring, which is used to determine appropriate drug doses, is critical in pharmacological therapy. In this study, we developed thermoresponsive chromatography columns with various cationic...
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Article
Open AccessTemperature-responsive mixed-mode column for the modulation of multiple interactions
In this study, mixed-mode chromatography columns have been investigated using multiple analyte interactions. A mixed-mode chromatography column was developed using poly(N-isopropylacrylamide) (PNIPAAm) brush-modi...
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Article
Open AccessTwo-dimensional temperature-responsive chromatography using a poly(N-isopropylacrylamide) brush-modified stationary phase for effective therapeutic drug monitoring
Therapeutic drug monitoring (TDM) is an effective pharmacological approach for controlling drug concentration in a patient’s serum. Herein, a new two-dimensional chromatography system was developed using two p...
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Article
Open AccessTemperature responsive chromatography for therapeutic drug monitoring with an aqueous mobile phase
Therapeutic drug monitoring is a key technology for effective pharmacological treatment. In the present study, a temperature-responsive chromatography column was developed for safe and simple therapeutic drug ...
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Article
Open AccessEffect of pore diameter on the elution behavior of analytes from thermoresponsive polymer grafted beads packed columns
Temperature-responsive chromatography using thermoresponsive polymers is innovative and can control analyte retention via column temperature. Analyte elution behavior in this type of chromatography depends on ...
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Article
Effective Separation for New Therapeutic Modalities Utilizing Temperature-responsive Chromatography
In recent years, drug discovery and therapeutics trends have shifted from a focus on small-molecule compounds to biopharmaceuticals, genes, cell therapy, and regenerative medicine. Therefore, new approaches an...
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Article
Open AccessSimultaneous analysis of multiple oligonucleotides by temperature-responsive chromatography using a poly(N-isopropylacrylamide)-based stationary phase
Oligonucleotide therapeutics have contributed remarkably to healthcare, being well suited for the treatment of intractable diseases that are difficult to approach using conventional drug modalities. However, a...
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Article
Open AccessAntibody drug separation using thermoresponsive anionic polymer brush modified beads with optimised electrostatic and hydrophobic interactions
Antibody drugs play an important role in biopharmaceuticals, because of the specificity for target biomolecules and reduction of side effects. Thus, separation and analysis techniques for these antibody drugs ...
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Article
Open AccessGreen analytical method for the simultaneous analysis of cytochrome P450 probe substrates by poly(N-isopropylacrylamide)-based temperature-responsive chromatography
High-performance liquid chromatography (HPLC) is the most common analytical method practiced in various fields and used for analysis of almost all drug compounds in the pharmaceutical industries. During drug d...
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Article
Open AccessPhenotypic traits of mesenchymal stem cell sheets fabricated by temperature-responsive cell culture plate: structural characteristics of MSC sheets
In most stem cell therapy strategies reported to date, stem cells are introduced to damaged tissue sites to repair and regenerate the original tissue structure and function. MSC therapeutic efficacies are inco...
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Article
Design of Tetra-arm PEG-crosslinked Thermoresponsive Hydrogel for 3D Cell Culture
We synthesized poly(N-isopropylacrylamide) gel containing cell-adhesive peptides. RGDS pendants, and crosslinked by a hydrophilic polymer. Since gelation occurs by simply mixing the polymers, cells can be simulta...
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Chapter and Conference Paper
Efficiency of Biological and Artificial Gills
An artificial gill is a device for the uptake of oxygen from water to air through a gas permeable membrane, driven by an oxygen partial pressure difference between the water and air. It enables humans to breat...
