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  1. Article

    Open Access

    Dynamic movement of the Golgi unit and its glycosylation enzyme zones

    Knowledge on the distribution and dynamics of glycosylation enzymes in the Golgi is essential for better understanding this modification. Here, using a combination of CRISPR/Cas9 knockin technology and super-r...

    Akihiro Harada, Masataka Kunii, Kazuo Kurokawa, Takuya Sumi… in Nature Communications (2024)

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  2. Article

    Open Access

    Single-molecule localization microscopy reveals STING clustering at the trans-Golgi network through palmitoylation-dependent accumulation of cholesterol

    Stimulator of interferon genes (STING) is critical for the type I interferon response to pathogen- or self-derived DNA in the cytosol. STING may function as a scaffold to activate TANK-binding kinase 1 (TBK1),...

    Haruka Kemmoku, Kanoko Takahashi, Kojiro Mukai, Toshiki Mori… in Nature Communications (2024)

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  3. No Access

    Article

    Hierarchical supramolecular structure comprising reduction-responsive DNA microspheres and semi-artificial glycopeptide-based micro-asters

    Herein, we describe the construction of a hierarchical supramolecular structure comprising reduction-responsive DNA microspheres and semi-artificial glycopeptide-based micro-asters, which are obtained from the...

    Ayaka Isogami, Sayuri L. Higashi, Bioru Okumura, Aya Shibata… in Polymer Journal (2023)

  4. No Access

    Article

    Recently developed glycosphingolipid probes and their dynamic behavior in cell plasma membranes as revealed by single-molecule imaging

    Glycosphingolipids, including gangliosides, are representative lipid raft markers that perform a variety of physiological roles in cell membranes. However, studies aimed at revealing their dynamic behavior in ...

    Kenichi G. N. Suzuki, Naoko Komura, Hiromune Ando in Glycoconjugate Journal (2023)

  5. No Access

    Article

    Fluorescent GD2 analog for single-molecule imaging

    Ganglioside GD2 is associated with the proliferation and migration of breast cancer cells. However, the precise role of GD2 is unclear because its tendency to form dynamic and transient domains in cell plasma ...

    Eriko Yamaguchi, Naoko Komura, Hide-Nori Tanaka, Akihiro Imamura… in Glycoconjugate Journal (2023)

  6. No Access

    Protocol

    Single-Molecule Imaging of Ganglioside Probes in Living Cell Plasma Membranes

    Gangliosides play a variety of physiological roles and are one of the most important lipid raft constituents. However, their dynamic behaviors have scarcely been investigated in living cells because of the lac...

    Kenichi G. N. Suzuki in Glycolipids (2023)

  7. Article

    Open Access

    AMPA receptors in the synapse turnover by monomer diffusion

    The number and subunit compositions of AMPA receptors (AMPARs), hetero- or homotetramers composed of four subunits GluA1–4, in the synapse is carefully tuned to sustain basic synaptic activity. This enables st...

    Jyoji Morise, Kenichi G. N. Suzuki, Ayaka Kitagawa… in Nature Communications (2019)

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  8. Article

    Open Access

    Dual-FRET imaging of IP3 and Ca2+ revealed Ca2+-induced IP3 production maintains long lasting Ca2+ oscillations in fertilized mouse eggs

    In most species, fertilization induces Ca2+ transients in the egg. In mammals, the Ca2+ rises are triggered by phospholipase Cζ (PLCζ) released from the sperm; IP3 generated by PLCζ induces Ca2+ release from the ...

    Toru Matsu-ura, Hideki Shirakawa, Kenichi G. N. Suzuki… in Scientific Reports (2019)

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  9. No Access

    Chapter

    Structural Biology of Glycans

    Cryo-electron microscopy, Single particle reconstruction, Drug design, Glycan-protein complex, Infectious diseases

    Chikara Sato, Koichi Kato, Yoshiki Yamaguchi… in Glycoscience: Basic Science to Applications (2019)

  10. No Access

    Article

    Super-long single-molecule tracking reveals dynamic-anchorage-induced integrin function

    Single-fluorescent-molecule imaging tracking (SMT) is becoming an important tool to study living cells. However, photobleaching and photoblinking (hereafter referred to as photobleaching/photoblinking) of the ...

    Taka A. Tsunoyama, Yusuke Watanabe, Junri Goto, Kazuma Naito… in Nature Chemical Biology (2018)

  11. No Access

    Chapter

    Unraveling of Lipid Raft Organization in Cell Plasma Membranes by Single-Molecule Imaging of Ganglioside Probes

    s are involved in a variety of physiological roles and particularly in the formation and function of lipid rafts in cell membranes. However, the dynamic behaviors of gangliosides have not been investigated i...

    Kenichi G. N. Suzuki, Hiromune Ando, Naoko Komura, Takahiro Fujiwara… in Glycobiophysics (2018)

  12. No Access

    Article

    Raft-based interactions of gangliosides with a GPI-anchored receptor

    Monitoring new fluorescent ganglioside analogs at a single-molecule level suggests that gangliosides associate dynamically with GPI-anchored protein monomers, transient homodimer rafts, and clustered signaling...

    Naoko Komura, Kenichi G N Suzuki, Hiromune Ando, Miku Konishi… in Nature Chemical Biology (2016)

  13. No Access

    Protocol

    Single-Molecule Imaging of Signal Transduction via GPI-Anchored Receptors

    Lipid rafts have been drawing extensive attention as a signaling platform. To investigate molecular interactions in lipid rafts, we often need to observe molecules in the plasma membranes of living cells becau...

    Kenichi G. N. Suzuki in Lipid Signaling Protocols (2016)

  14. No Access

    Reference Work Entry In depth

    Single Molecule Imaging

    Single-molecule imaging is a powerful tool to understand working mechanisms of glycoproteins and glycolipids in cell plasma membranes by overcoming intrinsically stochastic and inhomogeneous properties of biol...

    Kenichi G. N. Suzuki in Glycoscience: Biology and Medicine (2015)

  15. No Access

    Living Reference Work Entry In depth

    Single-Molecule Imaging

    Single-molecule imaging is a powerful tool to understand working mechanisms of glycoproteins and glycolipids in cell plasma membranes by overcoming intrinsically stochastic and inhomogeneous properties of biol...

    Kenichi G. N. Suzuki in Glycoscience: Biology and Medicine

  16. No Access

    Article

    Transient GPI-anchored protein homodimers are units for raft organization and function

    Single-molecule imaging reveals that GPI-anchored proteins in the plasma membrane form homodimer rafts via ectodomain protein interactions. Raft-lipid interactions stabilize higher order oligomers, which trigg...

    Kenichi G N Suzuki, Rinshi S Kasai, Koichiro M Hirosawa… in Nature Chemical Biology (2012)

  17. No Access

    Article

    Membrane molecules mobile even after chemical fixation

    Kenji A K Tanaka, Kenichi G N Suzuki, Yuki M Shirai, Shusaku T Shibutani… in Nature Methods (2010)