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  1. Article

    Open Access

    Integrated-gut-liver-on-a-chip platform as an in vitro human model of non-alcoholic fatty liver disease

    Non-alcoholic fatty liver disease (NAFLD) afflicts a significant percentage of the population; however, no effective treatments have yet been established because of the unsuitability of in vitro assays and ani...

    Jiandong Yang, Yoshikazu Hirai, Kei Iida, Shinji Ito in Communications Biology (2023)

  2. No Access

    Article

    An efficient simplified method for the generation of corneal epithelial cells from human pluripotent stem cells

    Corneal epithelial cells derived from human pluripotent stem cells (hPSCs) are an important cell source for preclinical models to test ophthalmic drugs. However, current differentiation protocols lack instruct...

    Rodi Abdalkader, Ken-ichiro Kamei in Human Cell (2022)

  3. No Access

    Chapter

    Nanofiber Extracellular Matrices in Regenerative Medicine

    Stem cells hold great potential for application in regenerative medicine and drug discovery. For these applications, stem cells need to be expanded by induced differentiation; these expanded stem cells need to...

    Ken-ichiro Kamei in Cell-Inspired Materials and Engineering (2021)

  4. No Access

    Protocol

    Microfluidic Image Cytometry for Single-Cell Phenoty** of Human Pluripotent Stem Cells

    A microfluidic human pluripotent stem cell (hPSC) array has been developed for robust and reproducible hPSC culture methods to assess chemically defined serum- and feeder-free culture conditions. This microflu...

    Yasumasa Mashimo, Ken-ichiro Kamei in Single Cell Protein Analysis (2015)

  5. No Access

    Article

    Chemically-defined scaffolds created with electrospun synthetic nanofibers to maintain mouse embryonic stem cell culture under feeder-free conditions

    Embryonic stem cells (ESCs) are useful resources for drug discovery, developmental biology and disease studies. Cellular microenvironmental cues play critical roles in regulating ESC functions, but it is chall...

    Li Liu, Qinghua Yuan, Jian Shi, **n Li, Dongju Jung, Li Wang in Biotechnology Letters (2012)

  6. No Access

    Protocol

    Microfluidic Image Cytometry

    Cell-based arrays offer powerful tools for genomics/proteomics and drug discovery, and are widely applicable for most cell lines. However, it is challenging to apply cell-based arrays for in vitro diagnosis du...

    Ken-ichiro Kamei, **g Sun, Hsian-Rong Tseng, Robert Damoiseaux in Cell-Based Microarrays (2011)

  7. No Access

    Article

    Cellular biosensing system for assessing immunomodulating effects on the inducible nitric oxide synthase (iNOS) cascade

    A cellular biosensing system has been constructed to assess the biological safety/toxicity of chemicals. Detection of nitric oxide (NO) by the cellular biosensing system was used as a readout for assessing the...

    Ken-ichiro Kamei, Tetsuya Haruyama, Masayasu Mie, Yasuko Yanagida in Biotechnology Letters (2003)