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Article
Open AccessModeling dry eye with an air–liquid interface in corneal epithelium-on-a-chip
Dry eye syndrome (DES) is a complex ocular condition characterized by an unstable tear film and inadequate tear production, leading to tissue damage. Despite its common occurrence, there is currently no compre...
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Article
Open AccessIntegrated-gut-liver-on-a-chip platform as an in vitro human model of non-alcoholic fatty liver disease
Non-alcoholic fatty liver disease (NAFLD) afflicts a significant percentage of the population; however, no effective treatments have yet been established because of the unsuitability of in vitro assays and ani...
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Article
Open AccessTumor-on-a-chip model for advancement of anti-cancer nano drug delivery system
Despite explosive growth in the development of nano-drug delivery systems (NDDS) targeting tumors in the last few decades, clinical translation rates are low owing to the lack of efficient models for evaluatin...
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Article
An efficient simplified method for the generation of corneal epithelial cells from human pluripotent stem cells
Corneal epithelial cells derived from human pluripotent stem cells (hPSCs) are an important cell source for preclinical models to test ophthalmic drugs. However, current differentiation protocols lack instruct...
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Article
Open AccessIn vitro culture at 39 °C during hepatic maturation of human ES cells facilitates hepatocyte-like cell functions
Hepatocyte-like cells derived from human pluripotent stem cells (hPSC-HLCs) offer an alternative to primary hepatocytes commonly used for drug screenings and toxicological tests. However, these cells do not ha...
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Article
Untargeted LC-MS Metabolomics for the Analysis of Micro-scaled Extracellular Metabolites from Hepatocytes
Metabolome analysis in micro physiological models is a challenge due to the low volume of the cell culture medium (CCM). Here, we report a LC-MS-based untargeted metabolomics protocol for the detection of hepa...
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Chapter
Nanofiber Extracellular Matrices in Regenerative Medicine
Stem cells hold great potential for application in regenerative medicine and drug discovery. For these applications, stem cells need to be expanded by induced differentiation; these expanded stem cells need to...
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Article
Three-dimensional cultured liver-on-a-Chip with mature hepatocyte-like cells derived from human pluripotent stem cells
Liver-on-a-Chip technology holds considerable potential for applications in drug screening and chemical-safety testing. To establish such platforms, functional hepatocytes are required; however, primary hepato...
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Article
3D printing of soft lithography mold for rapid production of polydimethylsiloxane-based microfluidic devices for cell stimulation with concentration gradients
Three-dimensional (3D) printing is advantageous over conventional technologies for the fabrication of sophisticated structures such as 3D micro-channels for future applications in tissue engineering and drug s...
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Protocol
Microfluidic Image Cytometry for Single-Cell Phenoty** of Human Pluripotent Stem Cells
A microfluidic human pluripotent stem cell (hPSC) array has been developed for robust and reproducible hPSC culture methods to assess chemically defined serum- and feeder-free culture conditions. This microflu...
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Article
Open AccessLocalized cell stimulation by nitric oxide using a photoactive porous coordination polymer platform
Functional cellular substrates for localized cell stimulation by small molecules provide an opportunity to control and monitor cell signalling networks chemically in time and space. However, despite improvemen...
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Article
Chemically-defined scaffolds created with electrospun synthetic nanofibers to maintain mouse embryonic stem cell culture under feeder-free conditions
Embryonic stem cells (ESCs) are useful resources for drug discovery, developmental biology and disease studies. Cellular microenvironmental cues play critical roles in regulating ESC functions, but it is chall...
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Protocol
Microfluidic Image Cytometry
Cell-based arrays offer powerful tools for genomics/proteomics and drug discovery, and are widely applicable for most cell lines. However, it is challenging to apply cell-based arrays for in vitro diagnosis du...
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Article
Integrated microfluidic devices for combinatorial cell-based assays
The development of miniaturized cell culture platforms for performing parallel cultures and combinatorial assays is important in cell biology from the single-cell level to the system level. In this paper we de...
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Article
A microfluidic platform for sequential ligand labeling and cell binding analysis
Develo** biochemical and cell biological assay for screening biomolecules, evaluating their characteristics in biological processes, and determining their pharmacological effects represents a key technology ...
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Article
Cellular biosensing system for assessing immunomodulating effects on the inducible nitric oxide synthase (iNOS) cascade
A cellular biosensing system has been constructed to assess the biological safety/toxicity of chemicals. Detection of nitric oxide (NO) by the cellular biosensing system was used as a readout for assessing the...
