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  1. Article

    Open Access

    Cdk1-mediated DIAPH1 phosphorylation maintains metaphase cortical tension and inactivates the spindle assembly checkpoint at anaphase

    Animal cells undergo rapid rounding during mitosis, ensuring proper chromosome segregation, during which an outward rounding force abruptly increases upon prometaphase entry and is maintained at a constant lev...

    Koutarou Nishimura, Yoshikazu Johmura, Katashi Deguchi… in Nature Communications (2019)

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  2. No Access

    Chapter and Conference Paper

    Structural Analysis of Long Single-Stranded RNA Molecules with Atomic Force Microscopy Imaging

    Characterization of the structure of long RNA molecules (>1 kb) is usually a time-consuming and tedious process. In this study, we have developed an imaging procedure for obtaining images of the extended secon...

    Jamie L. Gilmore, Aiko Yoshida… in 3rd International Multidisciplinary Micros… (2017)

  3. No Access

    Chapter and Conference Paper

    Structural/Functional Analyses of Protein-Nucleic Acid Interactions by AFM

    Here we review 20 years of investigations on the molecular mechanisms of protein-nucleic acid interactions using a combination of molecular biological and biochemical techniques and atomic force microscopy (AF...

    Kunio Takeyasu, Katashi Deguchi… in 3rd International Multidisciplinary Micros… (2017)

  4. No Access

    Protocol

    Analyses of Nuclear Proteins and Nucleic Acid Structures Using Atomic Force Microscopy

    Since the inception of atomic force microscopy (AFM) in 1986, the value of this technology for exploring the structure and biophysical properties of a variety of biological samples has been increasingly recogn...

    Jamie L. Gilmore, Aiko Yoshida, Hirohide Takahashi… in Nuclear Bodies and Noncoding RNAs (2015)