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Article
Open AccessMultiplexed transcriptome discovery of RNA-binding protein binding sites by antibody-barcode eCLIP
Ultraviolet crosslinking and immunoprecipitation (CLIP) methodologies enable the identification of RNA binding sites of RNA-binding proteins (RBPs). Despite improvements in the library preparation of RNA fragm...
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Article
Open AccessDistinct and shared functions of ALS-associated proteins TDP-43, FUS and TAF15 revealed by multisystem analyses
The RNA-binding protein (RBP) TAF15 is implicated in amyotrophic lateral sclerosis (ALS). To compare TAF15 function to that of two ALS-associated RBPs, FUS and TDP-43, we integrate CLIP-seq and RNA Bind-N-Seq ...
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Article
Divergent roles of ALS-linked proteins FUS/TLS and TDP-43 intersect in processing long pre-mRNAs
In this study, the authors compare the RNA binding patterns of FUS/TLS and TDP-43. Although these proteins regulate the processing of mostly distinct gene products, they do show concurrent regulation of a subs...
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Article
Long pre-mRNA depletion and RNA missplicing contribute to neuronal vulnerability from loss of TDP-43
The authors identify brain RNA targets for TDP-43, a RNA binding protein linked to ALS. RNAs derived from genes with very long introns were more affected by TDP-43 levels. TDP-43 also auto-regulated its own sy...
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Article
Comprehensive discovery of endogenous Argonaute binding sites in Caenorhabditis elegans
MicroRNAs are small RNAs involved in regulation of cognate mRNAs, but predicting their exact targets has been difficult. Using a cross-linking immunoprecipitation technique, a comprehensive examination of endo...
