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Protocol
Rattus norvegicus Spermatogenesis Colony-Forming Assays
Knowledge gaps persist on signaling pathways and metabolic states in germ cells sufficient to support spermatogenesis independent of a somatic environment. Consequently, methods to culture mammalian stem cells...
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Article
Slee** Beauty transposon mutagenesis in rat spermatogonial stem cells
We describe an experimental approach for generating mutant alleles in rat spermatogonial stem cells (SSCs) using Slee** Beauty (SB) transposon–mediated insertional mutagenesis. The protocol is based on mobiliza...
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Protocol
Rat Spermatogonial Stem Cell-Mediated Gene Transfer
More than 20 years have passed since the advent of genetic manipulation of the mouse germline using cultures of pluripotent embryonic stem cells. Still, despite remarkable successes in the mouse, the applicati...
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Article
Generating knockout rats by transposon mutagenesis in spermatogonial stem cells
Cultured rat spermatogonia, genetically modified via a transposon-based gene trap, are inserted into the testes of sterile rats and give rise to mutant progeny with many different genotypes.
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Protocol
Isolating Highly Pure Rat Spermatogonial Stem Cells in Culture
Methods are detailed for isolating highly pure populations of spermatogonial stem cells from primary cultures of testis cells prepared from 22- to 24-day-old rats. The procedure is based on the principle that ...
