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Open AccessCryo-EM structures of the human Elongator complex at work
tRNA modifications affect ribosomal elongation speed and co-translational folding dynamics. The Elongator complex is responsible for introducing 5-carboxymethyl at wobble uridine bases (cm5U34) in eukaryotic tRNA...
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Article
Open AccessKi-67 is necessary during DNA replication for fork protection and genome stability
The proliferation antigen Ki-67 has been widely used in clinical settings for cancer staging for many years, but investigations on its biological functions have lagged. Recently, Ki-67 has been shown to regula...
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Article
Open AccessStructure of the native γ-tubulin ring complex cap** spindle microtubules
Microtubule (MT) filaments, composed of α/β-tubulin dimers, are fundamental to cellular architecture, function and organismal development. They are nucleated from MT organizing centers by the evolutionarily co...
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Open AccessDifferentiation granules, a dynamic regulator of T. brucei development
Adaptation to a change of environment is an essential process for survival, in particular for parasitic organisms exposed to a wide range of hosts. Such adaptations include rapid control of gene expression thr...
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Open AccessProtein structure prediction with in-cell photo-crosslinking mass spectrometry and deep learning
While AlphaFold2 can predict accurate protein structures from the primary sequence, challenges remain for proteins that undergo conformational changes or for which few homologous sequences are known. Here we i...
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Article
Specific recognition and ubiquitination of translating ribosomes by mammalian CCR4–NOT
Translation affects messenger RNA stability and, in yeast, this is mediated by the Ccr4–Not deadenylation complex. The details of this process in mammals remain unclear. Here, we use cryogenic electron microsc...
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Open AccessReplisome-cohesin interactions provided by the Tof1-Csm3 and Mrc1 cohesion establishment factors
The chromosomal cohesin complex establishes sister chromatid cohesion during S phase, which forms the basis for faithful segregation of DNA replication products during cell divisions. Cohesion establishment is...
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Open AccessStructural analysis of an endogenous 4-megadalton succinyl-CoA-generating metabolon
The oxoglutarate dehydrogenase complex (OGDHc) participates in the tricarboxylic acid cycle and, in a multi-step reaction, decarboxylates α-ketoglutarate, transfers succinyl to CoA, and reduces NAD+. Due to it...
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Article
mRNA recognition and packaging by the human transcription–export complex
Newly made mRNAs are processed and packaged into mature ribonucleoprotein complexes (mRNPs) and are recognized by the essential transcription–export complex (TREX) for nuclear export1,2. However, the mechanisms o...
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Open AccessCommon mouse models of tauopathy reflect early but not late human disease
Mouse models that overexpress human mutant Tau (P301S and P301L) are commonly used in preclinical studies of Alzheimer’s Disease (AD) and while several drugs showed therapeutic effects in these mice, they were...
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Open AccessPublisher Correction: Visualizing translation dynamics at atomic detail inside a bacterial cell
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Open AccessVisualizing translation dynamics at atomic detail inside a bacterial cell
Translation is the fundamental process of protein synthesis and is catalysed by the ribosome in all living cells1. Here we use advances in cryo-electron tomography and sub-tomogram analysis2,3 to visualize the st...
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Article
The DNA-damage kinase ATR activates the FANCD2-FANCI clamp by priming it for ubiquitination
DNA interstrand cross-links are tumor-inducing lesions that block DNA replication and transcription. When cross-links are detected at stalled replication forks, ATR kinase phosphorylates FANCI, which stimulate...
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Proteasome-dependent truncation of the negative heterochromatin regulator Epe1 mediates antifungal resistance
Epe1 histone demethylase restricts H3K9-methylation-dependent heterochromatin, preventing it from spreading over, and silencing, gene-containing regions in fission yeast. External stress induces an adaptive re...
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Understudied proteins: opportunities and challenges for functional proteomics
Most research aiming at understanding the molecular foundations of life and disease has focused on a limited set of increasingly well-known proteins while the biological functions of many others remain poorly ...
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An open invitation to the Understudied Proteins Initiative
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Open AccessReliable identification of protein-protein interactions by crosslinking mass spectrometry
Protein-protein interactions govern most cellular pathways and processes, and multiple technologies have emerged to systematically map them. Assessing the error of interaction networks has been a challenge. Cr...
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Open AccessRetention time prediction using neural networks increases identifications in crosslinking mass spectrometry
Crosslinking mass spectrometry has developed into a robust technique that is increasingly used to investigate the interactomes of organelles and cells. However, the incomplete and noisy information in the mass...
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Article
Open AccessStructural basis for VPS34 kinase activation by Rab1 and Rab5 on membranes
The lipid phosphatidylinositol-3-phosphate (PI3P) is a regulator of two fundamental but distinct cellular processes, endocytosis and autophagy, so its generation needs to be under precise temporal and spatial ...
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Open AccessThe δ subunit and NTPase HelD institute a two-pronged mechanism for RNA polymerase recycling
Cellular RNA polymerases (RNAPs) can become trapped on DNA or RNA, threatening genome stability and limiting free enzyme pools, but how RNAP recycling into active states is achieved remains elusive. In Bacillus s...
