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Article
Open AccessIdentification and characterization of an Endo-glucanase secreted from cellulolytic Escherichia coli ZH-4
In the previous study, the cellulolytic Escherichia coli ZH-4 isolated from bovine rumen was found to show extracellular cellulase activity and could degrade cellulose in the culture. The goal of this work was to...
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Article
Open AccessThe phage T4 DNA ligase in vivo improves the survival-coupled bacterial mutagenesis
Microbial mutagenesis is an important avenue to acquire microbial strains with desirable traits for industry application. However, mutagens either chemical or physical used often leads narrow library pool due ...
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Article
Open AccessA strategy of gene overexpression based on tandem repetitive promoters in Escherichia coli
For metabolic engineering, many rate-limiting steps may exist in the pathways of accumulating the target metabolites. Increasing copy number of the desired genes in these pathways is a general method to solve ...