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Article
Open AccessCommon and distinct transcriptional signatures of mammalian embryonic lethality
The Deciphering the Mechanisms of Developmental Disorders programme has analysed the morphological and molecular phenotypes of embryonic and perinatal lethal mouse mutant lines in order to investigate the caus...
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Article
Open AccessHigh-throughput and quantitative genome-wide messenger RNA sequencing for molecular phenoty**
We present a genome-wide messenger RNA (mRNA) sequencing technique that converts small amounts of RNA from many samples into molecular phenotypes. It encompasses all steps from sample preparation to sequence a...
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Article
Correction: Corrigendum: The zebrafish reference genome sequence and its relationship to the human genome
Nature 496, 498–503 (2013); doi:10.1038/nature12111 In this Letter, five authors were inadvertently omitted: Sharmin Begum and Christine Lloyd from the Wellcome Trust Sanger Institute, and Christa Lanz, Günter...
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Article
Open AccessThe zebrafish reference genome sequence and its relationship to the human genome
A high-quality sequence assembly of the zebrafish genome reveals the largest gene set of any vertebrate and provides information on key genomic features, and comparison to the human reference genome shows that...
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Article
FRT-seq: amplification-free, strand-specific transcriptome sequencing
By using a reverse transcriptase for the bridge-amplification step on the Illumina Genome Analyzer, RNA conversion to cDNA and sequencing take place directly in the flowcell and yield highly accurate strand-sp...
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Article
Open AccessFinishing the finished human chromosome 22 sequence
Although the human genome sequence was declared complete in 2004, the sequence was interrupted by 341 gaps of which 308 lay in an estimated approximately 28 Mb of euchromatin. While these gaps constitute only ...
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Protocol
Subcellular Localization of Intracellular Human Proteins by Construction of Tagged Fusion Proteins and Transient Expression in COS-7 Cells
Identifying the subcellular compartment of a protein is an important step toward assigning protein function. Starting with a clone containing the open reading frame (ORF) of interest, it is possible to attach ...
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Article
Open AccessA genome annotation-driven approach to cloning the human ORFeome
We have developed a systematic approach to generating cDNA clones containing full-length open reading frames (ORFs), exploiting knowledge of gene structure from genomic sequence. Each ORF was amplified by PCR ...
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Protocol
YAC Library Storage and Transport
The development of the yeast artificial chromosome (YAC) cloning system (1) and the construction of YAC libraries with large numbers of genome equivalents (2–6) provided a major impetus to mammalian genome mappin...
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Protocol
YAC Library Screening
The storage of yeast artificial chromosome (YAC) libraries in ordered microtiter plates required a new approach to screening for clones containing specific DNA sequences. Screening libraries of some 60,000 clo...
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Protocol
YAC Library Screening
Yeast artificial chromosome (YAC) libraries stored in microtiter plates are available for screening as either complex PCR pools or hybridization filters generated from YACs gridded at high densities (see ...
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Article
Comparative analyses of the Dominant megacolon-SOX10 genomic interval in mouse and human
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Article
Characterization of the human synaptogyrin gene family
Genomic sequencing was combined with searches of databases for identification of active genes on human chromosome 22. A cosmid from 22q13, located in the telomeric vicinity of the PDGFB (platelet-derived growt...
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Protocol
YAC Library Screening I
The storage of yeast artificial chromosome (YAC) libraries in ordered microtiter plates required a new approach to screening for clones containing specific DNA sequences. Screening libraries of some 60,000 clo...
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Protocol
YAC Library Screening II
Yeast artificial chromosome (YAC) libraries stored in microtiter plates are available for screening as either complex PCR pools or hybridization filters generated from YACs gridded at high densities (see ...
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Protocol
YAC Library Storage and Transport
The large size of mammalian genomes necessitates the use of cloning vectors that will accommodate genomic DNA inserts of at least several hundred kilobases. The development of the yeast artificial chromosome (...
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Article
Genetic map** of 14 short tandem repeat polymorphisms on human chromosome 22
We have constructed a linkage map of 14 short tandem repeat polymorphisms (11 with heterozygosity > 70%) on the long arm of human chromosome 22 using 23 non-CEPH pedigrees. Twelve of the markers could be posit...
