29 Result(s)
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Article
Transgenic plants expressing two Bacillus thuringiensis toxins delay insect resistance evolution
Preventing insect pests from develo** resistance to Bacillus thuringiensis (Bt) toxins produced by transgenic crops is a major challenge for agriculture. Theoretical models suggest that plants containing two di...
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Bacillus thuringiensis protein production, signal transduction, and insect control in chemically inducible PR-1a/cry1Ab broccoli plants
In an effort to develop a chemically inducible system for insect management, we studied production of Cry1Ab Bacillus thuringiensis (Bt) protein and control of the diamondback moth (DBM), Plutella xylostella L., ...
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Building the sequence map of the human pan-genome
Here we integrate the de novo assembly of an Asian and an African genome with the NCBI reference human genome, as a step toward constructing the human pan-genome. We identified ∼5 Mb of novel sequences not presen...
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An optimized RNA amplification method for prokaryotic expression profiling analysis
DNA microarray technology has been extensively used for gene expression analysis of both eukaryotic and prokaryotic organisms. For eukaryotic gene expression profiling, the poly(A)-based reverse transcription ...
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Open AccessGenome sequence of foxtail millet (Setaria italica) provides insights into grass evolution and biofuel potential
Completion of genome sequences for the diploid Setaria italica reveals features of C4 photosynthesis that could enable improvement of the polyploid biofuel crop switchgrass (Panicum virgatum). The genetic basis o...
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Identification and molecular cytology analysis of cold tolerance introgression lines derived from Oryza sativa L. mating with O. rufipogon Griff.
A previously constructed introgression lines (ILs) population including 239 lines was used to evaluate the cold-tolerant ability. The ILs with strong cold tolerance which were identified in the present study w...
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Musca Domestica Larva Lectin Induces Apoptosis in BEL-7402 Cells Through a Ca2+/JNK-mediated Mitochondrial Pathway
Although Musca domestica larvae lectin (MLL) is able to inhibit cancer cell proliferation and to induce cancer cell apoptosis, the molecular mechanism(s) responsible for these processes remain elusive. In the ...
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Article
Effects of Chromium Methionine Supplementation on Growth Performance, Serum Metabolites, Endocrine Parameters, Antioxidant Status, and Immune Traits in Growing Pigs
The effects of dietary chromium methionine (CrMet) on growth performance, serum metabolites, endocrine parameters, antioxidant status, and immune traits in growing pigs were investigated. A total of 180 crossb...
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Enzymatic transformation of vina-ginsenoside R7 to rare notoginsenoside ST-4 using a new recombinant glycoside hydrolase from Herpetosiphon aurantiacus
An eco-friendly and convenient preparation method for notoginsenoside ST-4 has been established by completely transforming vina-ginsenoside R7 using a recombinant glycosidase hydrolyzing enzyme (HaGH03) from Herp...
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Article
Open AccessSequencing of allotetraploid cotton (Gossypium hirsutum L. acc. TM-1) provides a resource for fiber improvement
Two draft sequences of Gossypium hirsutum, the most widely cultivated cotton species, provide insights into genome structure, genome rearrangement, gene evolution and cotton fiber biology.
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Article
Effect of Dietary Selenomethionine Supplementation on Growth Performance, Tissue Se Concentration, and Blood Glutathione Peroxidase Activity in Kid Boer Goats
We used 240 kid Boer goats that were divided into six groups. The control group was fed a basal diet containing 0.05 mg of selenium (Se)/kg dry matter (DM). Trial groups received the basal diet supplemented wi...
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Effects of Graded Levels of Chromium Methionine on Performance, Carcass Traits, Meat Quality, Fatty Acid Profiles of Fat, Tissue Chromium Concentrations, and Antioxidant Status in Growing-Finishing Pigs
A 97-day feeding trial was conducted to investigate the effects of dietary chromium methionine (CrMet) on performance, carcass traits, meat quality, fatty acid profiles of fat, tissue chromium concentrations, ...
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Characterization of an IAA-glucose hydrolase gene TaTGW6 associated with grain weight in common wheat (Triticum aestivum L.)
In rice, the TGW6 gene determines grain weight and encodes a protein with indole-3-acetic acid (IAA)-glucose hydrolase activity. Its homolog in wheat, TaTGW6, is considered as a candidate gene related to grain de...
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Article
Determination of glutathione in apoptotic SMMC-7221 cells induced by xylitol selenite using capillary electrophoresis
To determine the glutathione (GSH) content in a human hepatoma cell line (SMMC-7221) treated with xylitol/selenite, providing a part of an investigation of its anti-cancer mechanisms.
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Article
Cloning and characterization of Tabas1-B1 gene associated with flag leaf chlorophyll content and thousand-grain weight and development of a gene-specific marker in wheat
The chloroplast photosystem of flag leaves contributes the largest proportion of photosynthates to grain in crops and consequently affects grain weight. The plant 2-Cys peroxiredoxin BAS1 is involved in chloro...
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Article
Pro-inflammatory Cytokines Are Involved in Fluoride-Induced Cytotoxic Potential in HeLa Cells
This study was designed to investigate the pro-inflammatory cytokines and their involvement in the cytotoxic potential of fluoride (F) in HeLa cells. HeLa cells were cultured with varying F concentrations (1–5...
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Article
A novel trichosanthin fusion protein with increased cytotoxicity to tumor cells
To evaluate the anti-tumor effects of trichosanthin after fusion with a cell penetrating peptide, heparin-binding peptide (HBP), derived from human heparin-binding EGF-like growth factor (HB-EGF).
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In vivo imaging of Ca2+ accumulation during cotton fiber initiation using fluorescent indicator YC3.60
Non-tip-focused Ca 2+ gradient indicated by genetically expressing a FRET-based calcium se...
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Article
A novel 33-bp insertion in the promoter of TaMFT-3A is associated with pre-harvest sprouting resistance in common wheat
TaMFT-3A on wheat chromosome 3AS, encoding a phosphatidyl ethanolamine-binding protein, was previously determined to be a candidate gene underlying the major quantitative trait locus (QTL) for pre-harvest sprouti...
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n-3 PUFA reduction caused by fabp2 deletion interferes with triacylglycerol metabolism and cholesterolhomeostasis in fish
Fatty acid-binding protein 2 (Fabp2), which is involved in the transport of long-chain fatty acids, is widely studied in mammals. Nevertheless, the role of this protein in teleost fish is mostly unknown. Here,...
