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Article
Structural determinants of SecB recognition by SecA in bacterial protein translocation
SecB is a bacterial chaperone involved in directing pre-protein to the translocation pathway by its specific interaction with the peripheral membrane ATPase SecA. The SecB-binding site on SecA is located at it...
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Article
Open AccessThe mutation network for the hemagglutinin gene from the novel influenza A (H1N1) virus
A mutation network for the hemagglutinin gene (HA) of the novel type A (H1N1) influenza virus was constructed. Sequence homology analysis indicated that one HA sequence type from the viruses mainly isolated from ...
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Article
Open AccessSubty** of type A influenza by sequencing the variable regions of HA gene specifically amplified with RT-PCR
The outbreak of a novel influenza A (H1N1) virus across the globe poses a threat to human health. It is of paramount importance to develop a rapid, reliable and inexpensive diagnostic procedure. Based on the b...
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Article
Open AccessStructure modeling and spatial epitope analysis for HA protein of the novel H1N1 influenza virus
In recent months, a novel influenza virus H1N1 broke out around the world. With bioinformatics technology, the 3D structure of HA protein was obtained, and the epitope residues were predicted with the method d...
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Article
Solution structure of the Magnaporthe oryzae avirulence protein AvrPiz-t
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Article
Enhanced enantioselectivity of a carboxyl esterase from Rhodobacter sphaeroides by directed evolution
The present work created an esterase variant from Rhodobacter sphaeroides (RspE) with enhanced selectivity in hydrolytic kinetic resolutions by directed evolution. A “model” substrate, methyl mandelate, was intro...
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Article
Open AccessThe structural basis of an NADP+-independent dithiol oxidase in FK228 biosynthesis
The disulfide bond is unusual in natural products and critical for thermal stability, cell permeability and bioactivity. DepH from Chromobacterium violaceum No. 968 is an FAD-dependent enzyme responsible for cata...
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Article
Open AccessMarked enhancement of Acinetobacter sp. organophosphorus hydrolase activity by a single residue substitution Ile211Ala
The activity of organophosphorus hydrolase (OPH) that catalyzes the hydrolysis of neurotoxic organophosphates (OPs) was reported to evolve from lactonase.
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Article
Open AccessCrystal structure of tyrosine decarboxylase and identification of key residues involved in conformational swing and substrate binding
Tyrosine decarboxylase (TDC) is a pyridoxal 5-phosphate (PLP)-dependent enzyme and is mainly responsible for the synthesis of tyramine, an important biogenic amine. In this study, the crystal structures of the...
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Article
Open AccessAn unusual UMP C-5 methylase in nucleoside antibiotic polyoxin biosynthesis
Polyoxin is a group of structurally-related peptidyl nucleoside antibiotics bearing C-5 modifications on the nucleoside skeleton. Although the structural diversity and bioactivity preference of polyoxin are, t...
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Article
l-Ribose isomerase and mannose-6-phosphate isomerase: properties and applications for l-ribose production
l-Ribose is a synthetic l-form monosaccharide. It is a building block of many novel nucleotide analog anti-viral drugs. Bio-production of l-ribose relies on a two-step reaction: (i) conve...
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Article
Structural basis of nonribosomal peptide macrocyclization in fungi
Unlike their bacterial counterparts, fungal nonribosomal peptide synthetases utilize a terminal condensation-like (CT) domain to form macrocycles, details of which are illuminated by structures of a CT domain and...
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Article
Open AccessGyrI-like proteins catalyze cyclopropanoid hydrolysis to confer cellular protection
GyrI-like proteins are widely distributed in prokaryotes and eukaryotes, and recognized as small-molecule binding proteins. Here, we identify a subfamily of these proteins as cyclopropanoid cyclopropyl hydrola...
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Article
Resistance-gene-directed discovery of a natural-product herbicide with a new mode of action
Bioactive natural products have evolved to inhibit specific cellular targets and have served as lead molecules for health and agricultural applications for the past century1–3. The post-genomics era has brought a...
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Article
Enhancement of ε-poly-l-lysine production by overexpressing the ammonium transporter gene in Streptomyces albulus PD-1
The antibacterial polymer ɛ-poly-l-lysine (ε-PL) has been widely used as a safe food preservative. As the synthesis of ε-PL requires a rich supply of nitrogen, the efficiency of nitrogen translocation and utiliza...
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Article
Structure-based design of agarase AgWH50C from Agarivorans gilvus WH0801 to enhance thermostability
AgWH50C, an exo-β-agarase of GH50 isolated from Agarivorans gilvus WH0801, plays a key role in the enzymatic production of neoagarobiose, which has great application prospect in the cosmetics and pharmaceutical i...
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Article
Open AccessArtificial cysteine-lipases with high activity and altered catalytic mechanism created by laboratory evolution
Engineering artificial enzymes with high activity and catalytic mechanism different from naturally occurring enzymes is a challenge in protein design. For example, many attempts have been made to obtain active...
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Article
Structural basis for stereoselective dehydration and hydrogen-bonding catalysis by the SAM-dependent pericyclase LepI
LepI is an S-adenosylmethionine (SAM)-dependent pericyclase that catalyses the formation of the 2-pyridone natural product leporin C. Biochemical characterization has shown that LepI can catalyse stereoselective ...
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Article
Open AccessStructure-guided engineering of a Thermobifida fusca cutinase for enhanced hydrolysis on natural polyester substrate
Cutinases could degrade insoluble polyester, including natural cutin and synthetic plastic. However, their turnover efficiency for polyester remains too low for industrial application. Herein, we report the 1....
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Open AccessCorrection to: Structure-guided engineering of a Thermobifida fusca cutinase for enhanced hydrolysis on natural polyester substrate
An amendment to this paper has been published and can be accessed via the original article.
