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Article
Recent advances in chemical protein synthesis: method developments and biological applications
The central dogma of modern biology underscores the pivotal roles proteins play in diverse biological processes, the study of which necessitates advanced methods to produce proteins with precision and versatil...
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Protocol
Removable Backbone Modification (RBM) Strategy for the Chemical Synthesis of Hydrophobic Peptides/Proteins
Chemical synthesis can provide hydrophobic proteins with natural or man-made modifications (e.g. S-palmitoylation, site-specific isotope labeling and mirror-image proteins) that are difficult to obtain through...
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Article
Open AccessSarcolipin alters SERCA1a interdomain communication by impairing binding of both calcium and ATP
Sarcolipin (SLN), a single-spanning membrane protein, is a regulator of the sarco-endoplasmic reticulum Ca2+-ATPase (SERCA1a). Chemically synthesized SLN, palmitoylated or not (pSLN or SLN), and recombinant wild-...
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Article
Open AccessChannel activity of mirror-image M2 proton channel of influenza A virus is blocked by achiral or chiral inhibitors
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Article
Recent advances in the chemical synthesis and semi-synthesis of poly-ubiquitin-based proteins and probes
Ubiquitination, a key and extensive posttranslational modification of proteins, has profound effects on a variety of physiological and pathological processes. The inherent complexity of ubiquitin conjugates ma...
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Article
Erratum: Chemically synthesized histone H2A Lys13 di-ubiquitination promotes binding of 53BP1 to nucleosomes
Correction to: Cell Research advance online publication 15 December 2017; doi:10.1038/cr.2017.157 During web production, there is an error on the website for the doi number. It should be doi:10.1038/cr.2017.15...
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Article
Chemically synthesized histone H2A Lys13 di-ubiquitination promotes binding of 53BP1 to nucleosomes
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Article
Chemical synthesis of membrane proteins by the removable backbone modification method
This protocol describes how to chemically synthesize membrane proteins through the installation of solubilizing removable backbone modification tags into hydrophobic transmembrane peptides. The implementation ...
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Article
Open AccessChemical synthesis of a two-photon-activatable chemokine and photon-guided lymphocyte migration in vivo
Chemokine-guided lymphocyte positioning in tissues is crucial for normal operation of the immune system. Direct, real-time manipulation and measurement of single-cell responses to chemokines is highly desired ...
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Article
Chemical synthesis of proteins using peptide hydrazides as thioester surrogates
This protocol provides a detailed procedure for the chemical synthesis of proteins through native chemical ligation of peptide hydrazides. The two crucial stages of this protocol are (i) the solid-phase synthe...
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Article
Chemical synthesis of a cyclotide via intramolecular cyclization of peptide O-esters
Cyclotides constitute a fascinating family of circular proteins containing ca. 30 amino acid residues. They have a unique cyclic cysteine knot topology and exhibit remarkable thermal, chemical and enzymatic st...
