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Article
Open AccessIntegrated transcriptomics and proteomics analysis reveals muscle metabolism effects of dietary Ulva lactuca and ulvan lyase supplementation in weaned piglets
Seaweeds, including the green Ulva lactuca, can potentially reduce competition between feed, food, and fuel. They can also contribute to the improved development of weaned piglets. However, their indigestible pol...
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Article
Open AccessAn in-planta comparative study of Plasmopara viticola proteome reveals different infection strategies towards susceptible and Rpv3-mediated resistance hosts
Plasmopara viticola, an obligate biotrophic oomycete, is the causal agent of one of the most harmful grapevine diseases, downy mildew. Within this pathosystem, much information is gathered on the host, as charact...
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Article
Distribution of cell-wall polysaccharides and proteins during growth of the hemp hypocotyl
The immuno-ultrastructural investigation localized cell-wall polysaccharides of bast fibers during hemp hypocotyl growth. Moreover, for the first time, the localization of a peroxidase and laccase is provided ...
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Article
Open AccessDoes long-term cadmium exposure influence the composition of pectic polysaccharides in the cell wall of Medicago sativa stems?
The heavy metal cadmium (Cd) accumulates in the environment due to anthropogenic influences. It is unessential and harmful to all life forms. The plant cell wall forms a physical barrier against environmental ...
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Article
Open AccessSpecialisation events of fungal metacommunities exposed to a persistent organic pollutant are suggestive of augmented pathogenic potential
The impacts of man-made chemicals, in particular of persistent organic pollutants, are multifactorial as they may affect the integrity of ecosystems, alter biodiversity and have undesirable effects on many org...
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Article
Open AccessGenetical genomics of quality related traits in potato tubers using proteomics
Recent advances in ~omics technologies such as transcriptomics, metabolomics and proteomics along with genotypic profiling have permitted the genetic dissection of complex traits such as quality traits in non-...
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Article
Open AccessInsights into the molecular regulation of monolignol-derived product biosynthesis in the growing hemp hypocotyl
Lignin and lignans are both derived from the monolignol pathway. Despite the similarity of their building blocks, they fulfil different functions in planta. Lignin strengthens the tissues of the plant, while lign...
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Article
Open AccessEnvironmental stress is the major cause of transcriptomic and proteomic changes in GM and non-GM plants
The approval of genetically modified (GM) crops is preceded by years of intensive research to demonstrate safety to humans and environment. We recently showed that in vitro culture stress is the major factor infl...
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Protocol
2D-DIGE in Proteomics
The two-dimensional difference gel electrophoresis method is a valuable approach for proteomics. The method, using cyanine fluorescent dyes, allows the co-migration of multiple protein samples in the same gel ...
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Article
Integrated proteomics and metabolomics to unlock global and clonal responses of Eucalyptus globulus recovery from water deficit
Water availability is well known for impacting productivity of Eucalyptus but comprehensive knowledge on cellular pathways involved in recovery and tolerance is scarce. In this context, we aimed to unveil putativ...
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Article
Open AccessEPMA-World Congress 2015
A1 Predictive and prognostic biomarker panel for targeted application of radioembolisation improving individual outcomes in hepatocellular carcinoma
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Article
Open AccessA proteome analysis of freezing tolerance in red clover (Trifolium pratense L.)
Improvement of freezing tolerance of red clover (Trifolium pratense L.) would increase its persistence under cold climate. In this study, we assessed the freezing tolerance and compared the proteome composition o...
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Article
Open AccessEffects of silver nanoparticles and ions on a co-culture model for the gastrointestinal epithelium
The increased incorporation of silver nanoparticles (Ag NPs) into consumer products makes the characterization of potential risk for humans and other organisms essential. The oral route is an important uptake ...
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Article
Open AccessElucidating how the saprophytic fungus Aspergillus nidulans uses the plant polyester suberin as carbon source
Lipid polymers in plant cell walls, such as cutin and suberin, build recalcitrant hydrophobic protective barriers. Their degradation is of foremost importance for both plant pathogenic and saprophytic fungi. R...
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Article
Open AccessMaize IgE binding proteins: each plant a different profile?
Allergies are nearly always triggered by protein molecules and the majority of individuals with documented immunologic reactions to foods exhibit IgE hypersensitivity reactions. In this study we aimed to under...
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Protocol
2DE Analysis of Forest Tree Proteins Using Fluorescent Labels and Multiplexing
Although proteomists working with gel-free methods are considering the gels as coming from the past, proteomics based on gels has still a lot of opportunities to offer and acquisition of images on which thousa...
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Article
Plant proteomics in India and Nepal: current status and challenges ahead
Plant proteomics has made tremendous contributions in understanding the complex processes of plant biology. Here, its current status in India and Nepal is discussed. Gel-based proteomics is predominantly utili...
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Chapter and Conference Paper
Protein expression in bovine mononuclear cells after stimulation with lipopolysaccharides and lipoteichoic acid: a proteomic approach
The aim of this project was to investigate the proteome of bovine mononuclear cells in physiological condition and after stimulation with different pathogen associated molecular patterns.
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Chapter and Conference Paper
Intrauterine treated lambs as a model for the study of Intrauterine Growth Restriction (IUGR) and consequent metabolic disorders
Developmental exposure to environmental and therapeutic chemicals, including excess androgens, has been reported to induce Intrauterine Growth Restriction (IUGR) and abnormal post-natal growth. It has now been...
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Article
Open AccessOptimization of iTRAQ labelling coupled to OFFGEL fractionation as a proteomic workflow to the analysis of microsomal proteins of Medicago truncatula roots
Shotgun proteomics represents an attractive technical framework for the study of membrane proteins that are generally difficult to resolve using two-dimensional gel electrophoresis. The use of iTRAQ, a set of ...
