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Chapter and Conference Paper
Physical Aspects of the Spin Labelling Technique
A brief review of the physical aspects of the spin labeling technique is given. Emphasis is put on the qualitative description of the phenomena which enable us to obtain information on molecular motion in biol...
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Chapter
Current Views on Boundary Lipids Deduced from Electron Spin Resonance Studies
In 1973 Jost and co-workers showed that a spin-labeled fatty acid, with the nitroxide ring near the ω-2 acyl terminal (spin label I), gives rise to a composite spectrum when incorporated into vesicles made wit...
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Article
Differentiation state-dependent surface mobilities of two forms of the neural cell adhesion molecule
The neural cell adhesion molecule (N-CAM) has been implicated in morphogenetic events during formation of the nervous system1,2. Three forms of N-CAM exist, all glycoprotein chains, of relative molecular masses 1...
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Chapter and Conference Paper
The Dynamics of Clathrin Coats in Living Cells Measured by Advanced Fluorescence Microscopy
Clathrin coated pits and coated vesicles are responsible for receptor-mediated endocytosis of a wide variety of ligands that bind at the cell surface (Anderson et al., 1977; Goldstein et al., 1985). Clathrin c...
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Chapter
Subcellular Cytofluorometry in Confocal Microscopy
The three-dimensional sampling capacity of confocal laser scanning microscopy can be used to analyze simultaneously the distribution of multiple proteins and nucleic acids. Confocal microscopes filter out most...
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Chapter
A Newly Identified Antigen Retention Compartment in the FSDC Precursor Dendritic Cell Line
Antigen uptake and presentation by dendritic cells (DC) occur at different stages of their maturation and are directed by certain cytokines (1–3). Phagocytosis (4) as well as mannose receptor-mediated endocyto...
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Chapter
Dendritic Cells from Mice Lacking the Invariant Chain Express High Levels of Membrane MHC Class II Molecules in Vivo
We investigated in H-2k mice bearing a genetically disrupted invariant chain (Ii) gene1, the MHC class II expression and antigen presentation ability of dendritic cells (DC) freshly purified from the spleen (SpDC...
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Chapter
Checkpoints and Functional Stages in DC Maturation
A major breakthrough in our understanding of the regulation of immune responses has been the discovery of a number of integrated functions of the dendritic cells (DC) in the immune system. To better define the...
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Chapter
Differential mRNA Expression in Untreated and TNF-α Elicited Murine Dendritic Cells Precursors
We have compared the pattern of gene expression in long term cultured precursor dendritic cells (DC), either untreated (immature) or cultured for two days in the presence of recombinant murine (rm)-TNFα1 (mature)...
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Article
Naked antigen-presenting molecules on dendritic cells
Antigen-presenting cells work to present peptides derived from exogenous and endogenous antigens to circulating T cells, sparking off an immune response. Dendritic cells are unique amongst antigen-presenting c...
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Article
IL-6 switches the differentiation of monocytes from dendritic cells to macrophages
Monocytes can give rise to either antigen presenting dendritic cells (DCs) or scavenging macrophages. This differentiation is initiated when monocytes cross the endothelium. But the regulation of DC and macrop...
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Article
ER–phagosome fusion defines an MHC class I cross-presentation compartment in dendritic cells
Induction of cytotoxic T-cell immunity requires the phagocytosis of pathogens, virus-infected or dead tumour cells by dendritic cells1. Peptides derived from phagocytosed antigens are then presented to CD8+ T lym...
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Article
Open AccessHighly efficient transduction of human plasmacytoid dendritic cells without phenotypic and functional maturation
Gene modified dendritic cells (DC) are able to modulate DC functions and induce therapeutic immunity or tolerance in an antigen-specific manner. Among the different DC subsets, plasmacytoid DC (pDC) are well k...
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Article
Open AccessIRAP-dependent endosomal T cell receptor signalling is essential for T cell responses
T cell receptor (TCR) activation is modulated by mechanisms such as TCR endocytosis, which is thought to terminate TCR signalling. Here we show that, upon internalization, TCR continues to signal from a set of...
