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Article
Intercrop** of aromatic plants in truffle orchards: short-term effect on extraradical truffle mycelium and aromatic plant growth
Intercrop** of truffle-producing trees with aromatic plants is used to improve profitability of truffle orchards during the initial 4–7 years. However, after that period the viability of this system is chall...
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Chapter
Mycorrhizae in Mediterranean Pine and Mixed Forests
Mediterranean forest dynamics are tightly linked to belowground fungal communities since fungi are involved in key ecosystem processes such as organic matter and nutrient cycling, water regulation, soil preser...
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Article
Glyphosate treatments for weed control affect early stages of root colonization by Tuber melanosporum but not secondary colonization
The cultivation of the ectomycorrhizal fungus Tuber melanosporum has considerably spread in recent years throughout the world. During the first years of truffle cultivation, weed control is a key practice to impr...
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Article
Seasonal dynamics of extraradical mycelium and mycorrhizas in a black truffle (Tuber melanosporum) plantation
Seasonal dynamics of black truffle (Tuber melanosporum) extraradical mycelium as well as the associated mycorrhizal community have been evaluated in a 16-year-old plantation with productive and non-productive tre...
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Article
Soil drying procedure affects the DNA quantification of Lactarius vinosus but does not change the fungal community composition
Drying soil samples before DNA extraction is commonly used for specific fungal DNA quantification and metabarcoding studies, but the impact of different drying procedures on both the specific fungal DNA quanti...
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Article
Diversity of ectomycorrhizal Thelephoraceae in Tuber melanosporum-cultivated orchards of Northern Spain
Truffles are edible hypogeous ascomycetes highly appreciated worldwide, especially the black truffle (Tuber melanosporum Vittad.). In recent decades, the cultivation of the black truffle has expanded across the M...
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Chapter
Tools to Trace Truffles in Soil
Different methodological tools have been developed to trace truffles in soil, from fruiting body collections and mycorrhiza morphoty** to recent high-sensitive, molecular techniques able to detect specific t...
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Article
Ectomycorrhizal fungus diversity and community structure with natural and cultivated truffle hosts: applying lessons learned to future truffle culture
Since the first truffle plantations were established in France, Italy and other parts in the world, many studies have been carried out to improve their productivity and sustainability. Success of plantations i...
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Chapter
Erratum to Chapter 8: How Ectomycorrhizae Structures Boost the Root System?
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Chapter
How Ectomycorrhizae Structures Boost the Root System?
Mycorrhizae are classic examples to explain the mutualistic interaction between two different organisms in nature: the roots of a vascular plant and a fungus. Both species establish a permanent relationship, t...
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Article
Seasonal dynamics of Boletus edulis and Lactarius deliciosus extraradical mycelium in pine forests of central Spain
The annual belowground dynamics of extraradical soil mycelium and sporocarp production of two ectomycorrhizal fungi, Boletus edulis and Lactarius deliciosus, have been studied in two different pine forests (Pinar...
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Article
Quantification of extraradical mycelium of Tuber melanosporum in soils from truffle orchards in northern Spain
Quantification of extraradical mycelium of black truffle (Tuber melanosporum) has been carried out in a natural truffle ground and in seven truffle orchards (around 20 years old) established in Tierra Estella and...
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Article
Quantification of extraradical soil mycelium and ectomycorrhizas of Boletus edulis in a Scots pine forest with variable sporocarp productivity
The availability of most edible ectomycorrhizal mushrooms depends on their natural fructification. Sporocarp formation of these fungi is linked to habitat characteristics and climate conditions, but these data...
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Article
Intraspecific variability of Lactarius deliciosus isolates: colonization ability and survival after cold storage
Intraspecific variability in root colonization, extraradical growth pattern, and survival after cold storage of Lactarius deliciosus isolates was determined in pure culture conditions using Pinus pinaster as a ho...
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Article
Field persistence of the edible ectomycorrhizal fungus Lactarius deliciosus: effects of inoculation strain, initial colonization level, and site characteristics
Pinus pinea plants were inoculated with different strains of the edible ectomycorrhizal fungus Lactarius deliciosus. The inoculated plants were established in six experimental plantations in two sites located in...
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Article
Mycorrhizal synthesis between Boletus edulis species complex and rockroses (Cistus sp.)
Ectomycorrhizas of Boletus aereus, Boletus edulis, and Boletus reticulatus were synthesized with Cistus sp. under laboratory conditions using synthesis tubes filled with a mixture of sterilized peat-vermiculite a...
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Article
The influence of the ectomycorrhizal fungus Rhizopogon subareolatus on growth and nutrient element localisation in two varieties of Douglas fir (Pseudotsuga menziesii var. menziesii and var. glauca) in response to manganese stress
Acidification of forest ecosystems leads to increased plant availability of the micronutrient manganese (Mn), which is toxic when taken up in excess. To investigate whether ectomycorrhizas protect against exce...
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Article
Tracking mycorrhizas and extraradical mycelium of the edible fungus Lactarius deliciosus under field competition with Rhizopogon spp
The objective of this study is to evaluate the field persistence of the edible ectomycorrhizal fungus Lactarius deliciosus in competition with two ubiquitous soil fungi. Couples of plants inoculated with either L...
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Article
Influence of the fertilisation method in controlled ectomycorrhizal inoculation of two Mediterranean pines
The influence of the fertilisation method: soluble (SF) vs. slow-release fertiliser (SRF) and of inoculation with Laccaria laccata (Scop.) Fr., Pisolithus tinctorius (Pers.) Coker & Couch and Melanogaster ambiguu...
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Article
Evaluation of mycelial inocula of edible Lactarius species for the production of Pinus pinaster and P. sylvestris mycorrhizal seedlings under greenhouse conditions
Different methods to inoculate seedlings of Pinus pinaster and P. sylvestris with edible Lactarius species under standard greenhouse conditions were evaluated. Fungal inoculations were performed both under pure c...
