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Article
Open AccessCyclic AMP signaling in Dictyostelium promotes the translocation of the copine family of calcium-binding proteins to the plasma membrane
Copines are calcium-dependent phospholipid-binding proteins found in many eukaryotic organisms and are thought to be involved in signaling pathways that regulate a wide variety of cellular processes. Copines a...
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Article
Phaged and confused by biofilm matrix
Bacterial biofilms fabricate an extracellular amyloid fibre network that intimately links cells together and inhibits the ability of bacteriophages to penetrate the biofilm.
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Protocol
Spontaneous and Experimental Metastasis Models: Nude Mice
Immunodeficient mice are widely used for cancer research as they can provide an in vivo system in which to study the tumorigenicity and metastatic potential of human cancer cells. The athymic or “nude” mouse h...
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Article
Open AccessThe role of MMP-1 in breast cancer growth and metastasis to the brain in a xenograft model
Brain metastasis is an increasingly common complication for breast cancer patients; approximately 15– 30% of breast cancer patients develop brain metastasis. However, relatively little is known about how these...
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Article
Open AccessRepair and Reconstruction of a Resected Tumor Defect Using a Composite of Tissue Flap–Nanotherapeutic–Silk Fibroin and Chitosan Scaffold
A multifaceted strategy using a composite of anti-cancer nanotherapeutic and natural biomaterials silk fibroin (SF) and chitosan (CS) blend scaffolds was investigated for the treatment of a tissue defect post-...
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Article
Open AccessRac1 and Rac3 isoform activation is involved in the invasive and metastatic phenotype of human breast cancer cells
The metastatic progression of cancer is a direct result of the disregulation of numerous cellular signaling pathways, including those associated with adhesion, migration, and invasion. Members of the Rac famil...
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Article
Open AccessPrime–boost vaccination with plasmid and adenovirus gene vaccines control HER2/neu+metastatic breast cancer in mice
Once metastasis has occurred, the possibility of completely curing breast cancer is unlikely, particularly for the 30 to 40% of cancers overexpressing the gene for HER2/neu. A vaccine targeting p185, the protein ...
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Article
Chemokine receptor CXCR4 expression in breast cancer as a potential predictive marker of isolated tumor cells in bone marrow
Interactions between the CXCR4 chemokine receptor in breast cancer cells and the ligand CXCL12/SDF-1α are thought to play an important role in breast cancer metastases. In this pilot study, CXCR4 expression al...
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Protocol
Clinically Relevant Metastatic Breast Cancer Models to Study Chemosensitivity
Metastatic disease, notably to the lungs, liver, bone, and brain, is the most common cause of death from breast cancer, despite advances in surgical and clinical management. Two basic principles govern the pro...
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Article
Vascular endothelial growth factor expression promotes the growth of breast cancer brain metastases in nude mice
Patients with breast cancer brain metastases cannot be cured and have a poor prognosis, with a median survival time of six months after diagnosis, despite developments in diagnostic and therapeutic modalities....
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Article
The Role of the Guanine Nucleotide Exchange Factor Tiam1 in Cellular Migration, Invasion, Adhesion and Tumor Progression
While advances in molecular genetics have provided new insights into molecular alterations that lead to the development of many tumors, including breast carcinoma, the genetic and epigenetic alterations that r...
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Article
Selection of more aggressive variants of the GI101A human breast cancer cell line: A model for analyzing the metastatic phenotype of breast cancer
In vivo models utilizing orthotopic injection of tumor cells into nude mice have proven valuable for the study of metastasis. However, breast cancers are among the more difficult of human tumors to grow in immuno...
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Article
DNA vaccination against neu reduces breast cancer incidence and metastasis in mice
The gene for HER2/neu is overexpressed in 30–40% of breast and ovarian cancers, and this overexpression correlates with increased metastasis and poor prognosis. The HER2/neu gene product, a transmembrane protein ...
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Protocol
Xenograft Models in Immunodeficient Animals
The growth of metastases is the end result of a multistep process in which cancer cells invade through basement membranes, extravasate into bloodstream or lymphatic vessels, survive transit in the circulation,...
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Article
Increased levels of α6 integrins are associated with the metastatic phenotype of human breast cancer cells
Integrins play an important role in interactions between cells and the extracellular matrix, and thus have a potential role in metastasis. Expression levels of α6, β1 and β4 integrin sub-units were measured in...
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Article
Overexpression of both p185c-erbB2 and p170mdr-1 renders breast cancer cells highly resistant to taxol
We recently found that overexpression of p185c-erbB2 in c-erbB2 transfected MDA-MB-435 breast cancer cells (435.eB transfectants) confers a 5–9-fold increase in Taxol resistance. To examine whether Taxol resistan...
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Article
Dominant-negative CREB inhibits tumor growth and metastasis of human melanoma cells
The ATF/CREB family of eukaryotic transcription factors contain the bZIP structural motif and mediate their transcriptional activities via heterodimerization with ATF and AP-1 family members. Quenching of CREB...
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Article
Inhibition of colony formation in agarose of metastatic human breast carcinoma and melanoma cells by synthetic glycoamine analogs
We studied the influence of 10 synthetic glycoamine analogs on colony formation in 0.3 and 0.9% agarose by metastatic human breast carcinoma (MDA-MB-435) and melanoma (TXM-13) cells. Nine synthetic analogs sig...
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Article
Metastasis from human breast cancer cell lines
Immunodeficient animals, principally nude mice, when used in appropriately designed studies have been shown to be useful for the experimental analysis of human breast cancer metastasis. As with many other huma...
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Article
Characterization of the invasive and metastatic phenotype in human renal cell carcinoma
The purpose of these studies was to identify some characteristics of metastatic cells and deficiencies of non-metastatic cells in the heterogeneous SN12 human renal cell carcinoma. The SN12 parental line and s...
