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Protocol
Clonal Analysis of Human Dendritic Cell Progenitors Using a Stromal Cell Culture
Dendritic cells (DCs) are a heterogenous population of professional antigen-presenting cells that play an “educator” role in immunity. Multiple DC subsets collaboratively initiate and orchestrate innate and ad...
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Article
Open AccessReduced calcium levels and accumulation of abnormal insulin granules in stem cell models of HNF1A deficiency
Mutations in HNF1A cause Maturity Onset Diabetes of the Young (HNF1A-MODY). To understand mechanisms of β-cell dysfunction, we generated stem cell-derived pancreatic endocrine cells with hypomorphic mutations in
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Article
Open AccessSingle cell RNA-Seq reveals pre-cDCs fate determined by transcription factor combinatorial dose
Classic dendritic cells (cDCs) play a central role in the immune system by processing and presenting antigens to activate T cells, and consist of two major subsets: CD141+ cDC (cDC1) and CD1c+ cDC (cDC2). A popul...
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Article
Erratum: Lineage specification of human dendritic cells is marked by IRF8 expression in hematopoietic stem cells and multipotent progenitors
Nat. Immunol.; doi:10.1038/ni.3789; corrected online 5 July 2017 In the version of this article initially published online, the flow cytometric dots were missing in the middle plot of the leftmost column in Fi...
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Article
Lineage specification of human dendritic cells is marked by IRF8 expression in hematopoietic stem cells and multipotent progenitors
Liu and colleagues show that specification of the dendritic-cell lineage occurs in parallel with specification of the myeloid and lymphoid lineages in or around the hematopoietic-stem-cell stage, starting as a...
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Article
Defining human dendritic cell progenitors by multiparametric flow cytometry
This protocol describes flow cytometry panels that can be used to analyze and isolate dendritic cell (DC) progenitors including granulocyte, monocyte and DC progenitor cells; monocyte and DC progenitor cells; ...
